ArticleGene therapy2007
Lentivirus-mediated gene transfer to the rat, ovine and human cornea.
Article in Gene therapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Review
- Article
- Corneal gene therapy: basic science and translational perspective.The ocular surface · 2013Review
- Diminished Memory T-Cell Expansion Due to Delayed Kinetics of Antigen Expression by Lentivectors.PloS one · 2013Article
- Ocular gene delivery using lentiviral vectors.Gene therapy · 2012Review
- Gene therapy in the cornea: 2005--present.Progress in retinal and eye research · 2012Review
- Anti-apoptotic gene therapy prolongs survival of corneal endothelial cells during storage.Gene therapy · 2011Article
- Corneal endothelial cells are protected from apoptosis by gene therapy.Human gene therapy · 2011Article
- Refinement of lentiviral vector for improved RNA processing and reduced rates of self inactivation repair.BMC biotechnology · 2009Article
- Efficient lentiviral gene transfer into corneal stroma cells using a femtosecond laser.Gene therapy · 2009Article
- VIP and VIP gene silencing modulation of differentiation marker N-cadherin and cell shape of corneal endothelium in human corneas ex vivo.Investigative ophthalmology & visual science · 2008Article
- Overexpression of a mutant form of TGFBI/BIGH3 induces retinal degeneration in transgenic mice.Molecular vision · 2008Article
- Different tropism of adenoviruses and adeno-associated viruses to corneal cells: implications for corneal gene therapy.Molecular vision · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene therapy of the cornea shows promise for modulating corneal transplant rejection but the most appropriate vector for gene transfer has yet to be determined. We investigated a lentiviral vector (LV) for its ability to transduce corneal endothelium. A lentivector expressing enhanced yellow fluorescent protein (eYFP) under the control of the Simian virus type 40 early promoter (LV-SV40-eYFP) transduced 80-90% of rat, ovine and human corneal endothelial cells as detected by fluorescence microscopy. The kinetics of gene expression varied among species, with ovine corneal endothelium showing a relative delay in detectable reporter gene expression compared with the rat or human corneal endothelium. Vectors containing the myeloproliferative sarcoma virus promoter or the phosphoglycerate kinase promoter were not significantly more effective than LV-SV40-eYFP. The stability of eYFP expression in rat and ovine corneas following ex vivo transduction of the donor cornea was assessed following orthotopic corneal transplantation. Following transduction ex vivo, eYFP expression was maintained in corneal endothelial cells for at least 28 days after corneal transplantation in the sheep and >60 days in the rat. Thus, rat, ovine and human corneal endothelial cells were efficiently transduced by the LV, and gene expression appeared stable over weeks in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.