ArticleNucleic acids research2007
Group II intron in Bacillus cereus has an unusual 3' extension and splices 56 nucleotides downstream of the predicted site.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Group II Introns: Flexibility and Repurposing.Frontiers in molecular biosciences · 2022Article
- The Ll.LtrB intron from Lactococcus lactis excises as circles in vivo: insights into the group II intron circularization pathway.RNA (New York, N.Y.) · 2015Article
- Evolution of group II introns.Mobile DNA · 2015Article
- Survey of chimeric IStron elements in bacterial genomes: multiple molecular symbioses between group I intron ribozymes and DNA transposons.Nucleic acids research · 2014Article
- Novel RNA structural features of an alternatively splicing group II intron from Clostridium tetani.RNA (New York, N.Y.) · 2014Article
- Predicted group II intron lineages E and F comprise catalytically active ribozymes.RNA (New York, N.Y.) · 2013Article
- Diversity, mobility, and structural and functional evolution of group II introns carrying an unusual 3' extension.BMC research notes · 2011Article
- Recurrent insertion of 5'-terminal nucleotides and loss of the branchpoint motif in lineages of group II introns inserted in mitochondrial preribosomal RNAs.RNA (New York, N.Y.) · 2011Article
- The tertiary structure of group II introns: implications for biological function and evolution.Critical reviews in biochemistry and molecular biology · 2010Review
- A conserved 3' extension in unusual group II introns is important for efficient second-step splicing.Nucleic acids research · 2009Article
- Group II introns in eubacteria and archaea: ORF-less introns and new varieties.RNA (New York, N.Y.) · 2008Article
- Survey of group I and group II introns in 29 sequenced genomes of the Bacillus cereus group: insights into their spread and evolution.Nucleic acids research · 2008Article
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4 authors.
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Abstract
All group II introns known to date fold into six functional domains. However, we recently identified an intron in Bacillus cereus ATCC 10987, B.c.I4, that splices 56 nt downstream of the expected 3' splice site in vivo (Tourasse et al. 2005, J. Bacteriol., 187, 5437-5451). In this study, we confirmed by ribonuclease protection assay that the 56-bp segment is part of the intron RNA molecule, and computational prediction suggests that it might form a stable stem-loop structure downstream of domain VI. The splicing of B.c.I4 was further investigated both in vivo and in vitro. Lariat formation proceeded primarily by branching at the ordinary bulged adenosine in domain VI without affecting the fidelity of splicing. In addition, the splicing efficiency of the wild-type intron was better than that of a mutant construct deleted of the 56-bp 3' extension. These results indicate that the intron has apparently adapted to the extra segment, possibly through conformational adjustments. The extraordinary group II intron B.c.I4 harboring an unprecedented extra 3' segment constitutes a dramatic example of the flexibility and adaptability of group II introns.
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