ArticleProceedings of the National Academy of Sciences of the United States of America2007
Adenovirus-mediated gene delivery into mouse spermatogonial stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 66 citations in OpenAlex.
- Male germ cell technologies in large animals.The Journal of reproduction and development · 2026Review
- Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Restoring fertility with a lipid nanoparticle-mRNA platform: A new era in male germline therapy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A Simple and Safe Protocol for Intra-Testicular Gene Delivery in Neonatal Mice Using a Convenient Isoflurane-Based Anesthesia System.Biotech (Basel (Switzerland)) · 2025Article
- Sperm and offspring production in a nonobstructive azoospermia mouse model via testicular mRNA delivery using lipid nanoparticles.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Glutamine protects mouse spermatogonial stem cells against NOX1-derived ROS for sustaining self-renewal division in vitro.Development (Cambridge, England) · 2023Article
- Generation of Transgenic Sperm Expressing GFP by Lentivirus Transduction of Spermatogonial Stem Cells In Vivo in Cynomolgus Monkeys.Veterinary sciences · 2023Article
- Adenovirus-mediated gene delivery restores fertility in congenitally infertile female mice.The Journal of reproduction and development · 2022Article
- Gene therapy for female infertility: A farfetched dream or reality?Cell reports. Medicine · 2022Article
- Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system.Scientific reports · 2021Article
- An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia.Genes & development · 2021Article
- Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation.Frontiers in physiology · 2021Article
- Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein.Stem cell reports · 2020Article
- ROS amplification drives mouse spermatogonial stem cell self-renewal.Life science alliance · 2019Article
- Biology and manipulation technologies of male germline stem cells in mammals.Reproductive medicine and biology · 2018Review
- In Vivo Genetic Manipulation of Spermatogonial Stem Cells and Their Microenvironment by Adeno-Associated Viruses.Stem cell reports · 2018Article
- TALEN-mediated gene targeting in porcine spermatogonia.Molecular reproduction and development · 2018Article
- Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring.Cell research · 2017Article
- Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal.Genes & development · 2016Article
- Mouse Spermatogenesis Requires Classical and Nonclassical Testosterone Signaling.Biology of reproduction · 2016Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spermatogonial stem cells represent a self-renewing population of spermatogonia, and continuous division of these cells supports spermatogenesis throughout the life of adult male animals. Previous attempts to introduce adenovirus vectors into spermatogenic cells, including spermatogonial stem cells, have failed to yield evidence of infection, suggesting that male germ cells may be resistant to adenovirus infection. In this study we show the feasibility of transducing spermatogonial stem cells by adenovirus vectors. When testis cells from ROSA26 Cre reporter mice were incubated in vitro with a Cre-expressing adenovirus vector, Cre-mediated recombination occurred at an efficiency of 49-76%, and the infected spermatogonial stem cells could reinitiate spermatogenesis after transplantation into seminiferous tubules of infertile recipient testes. No evidence of germ-line integration of adenovirus vector could be found in offspring from infected stem cells that underwent Cre-mediated recombination, which suggests that the adenovirus vector infected the cells but did not stably integrate into the germ line. Nevertheless, these results suggest that adenovirus may inadvertently integrate into the patient's germ line and indicate that there is no barrier to adenovirus infection in spermatogonial stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.