ArticlePLoS biology2007
Exon silencing by UAGG motifs in response to neuronal excitation.
Article in PLoS biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.
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Who cites it
53 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.
- Meta-analysis of activated neurons reveals dynamic regulation of diverse classes of alternative splicing.Genome research · 2025Pooled it
- Stabilizing a mammalian RNA thermometer confers neuroprotection in subarachnoid hemorrhage.Nature communications · 2025Article
- An intronic RNA element modulates Factor VIII exon-16 splicing.Nucleic acids research · 2024Article
- Calcium and activity-dependent signaling in the developing cerebral cortex.Development (Cambridge, England) · 2022Review
- Alternative splicing modulation by G-quadruplexes.Nature communications · 2022Article
- hnRNP A1 in RNA metabolism regulation and as a potential therapeutic target.Frontiers in pharmacology · 2022Review
- Do Fragile X Syndrome and Other Intellectual Disorders Converge at Aberrant Pre-mRNA Splicing?Frontiers in psychiatry · 2021Article
- MeCP2 gates spatial learning-induced alternative splicing events in the mouse hippocampus.Molecular brain · 2020Article
- FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism.Cell reports · 2020Article
- Regulation of Neuronal Differentiation, Function, and Plasticity by Alternative Splicing.Annual review of cell and developmental biology · 2018Review
- Identification and characterization of two novel alternatively spliced E2F1 transcripts in the rat CNS.Molecular and cellular neurosciences · 2018Article
- Repurposing Dantrolene for Long-Term Combination Therapy to Potentiate Antisense-Mediated DMD Exon Skipping in the mdx Mouse.Molecular therapy. Nucleic acids · 2018Article
- Global analysis of physical and functional RNA targets of hnRNP L reveals distinct sequence and epigenetic features of repressed and enhanced exons.RNA (New York, N.Y.) · 2015Article
- RNA ligation in neurons by RtcB inhibits axon regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Histone hyperacetylation and exon skipping: a calcium-mediated dynamic regulation in cardiomyocytes.Nucleus (Austin, Tex.) · 2015Article
- Calcium-mediated histone modifications regulate alternative splicing in cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Emerging mechanisms and consequences of calcium regulation of alternative splicing in neurons and endocrine cells.Cellular and molecular life sciences : CMLS · 2013Review
- Depolarization alters phenotype, maintains plasticity of predifferentiated mesenchymal stem cells.Tissue engineering. Part A · 2013Article
- hnRNP A1 and secondary structure coordinate alternative splicing of Mag.RNA (New York, N.Y.) · 2013Article
- Alternative splicing: functional diversity among voltage-gated calcium channels and behavioral consequences.Biochimica et biophysica acta · 2013Review
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2 authors at 1 institution in 1 country.
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Abstract
Alternative pre-mRNA splicing plays fundamental roles in neurons by generating functional diversity in proteins associated with the communication and connectivity of the synapse. The CI cassette of the NMDA R1 receptor is one of a variety of exons that show an increase in exon skipping in response to cell excitation, but the molecular nature of this splicing responsiveness is not yet understood. Here we investigate the molecular basis for the induced changes in splicing of the CI cassette exon in primary rat cortical cultures in response to KCl-induced depolarization using an expression assay with a tight neuron-specific readout. In this system, exon silencing in response to neuronal excitation was mediated by multiple UAGG-type silencing motifs, and transfer of the motifs to a constitutive exon conferred a similar responsiveness by gain of function. Biochemical analysis of protein binding to UAGG motifs in extracts prepared from treated and mock-treated cortical cultures showed an increase in nuclear hnRNP A1-RNA binding activity in parallel with excitation. Evidence for the role of the NMDA receptor and calcium signaling in the induced splicing response was shown by the use of specific antagonists, as well as cell-permeable inhibitors of signaling pathways. Finally, a wider role for exon-skipping responsiveness is shown to involve additional exons with UAGG-related silencing motifs, and transcripts involved in synaptic functions. These results suggest that, at the post-transcriptional level, excitable exons such as the CI cassette may be involved in strategies by which neurons mount adaptive responses to hyperstimulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.