ArticleProceedings of the National Academy of Sciences of the United States of America1992
Identification of act2, an essential gene in the fission yeast Schizosaccharomyces pombe that encodes a protein related to actin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 86 citations in OpenAlex.
- The Diverse Family of Arp2/3 Complexes.Trends in cell biology · 2017Review
- Review
- Actin organization and dynamics in filamentous fungi.Nature reviews. Microbiology · 2011Review
- Forward genetics in Candida albicans that reveals the Arp2/3 complex is required for hyphal formation, but not endocytosis.Molecular microbiology · 2010Article
- btn1 affects endocytosis, polarization of sterol-rich membrane domains and polarized growth in Schizosaccharomyces pombe.Traffic (Copenhagen, Denmark) · 2008Article
- The ARP2/3 complex: an actin nucleator comes of age.Nature reviews. Molecular cell biology · 2006Review
- Sequence and comparative genomic analysis of actin-related proteins.Molecular biology of the cell · 2005Article
- Article
- Actin-based motility of intracellular microbial pathogens.Microbiology and molecular biology reviews : MMBR · 2001Review
- Interactions among a fimbrin, a capping protein, and an actin-depolymerizing factor in organization of the fission yeast actin cytoskeleton.Molecular biology of the cell · 2001Article
- The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.The Journal of cell biology · 1997Article
- Structure, subunit topology, and actin-binding activity of the Arp2/3 complex from Acanthamoeba.The Journal of cell biology · 1997Article
- The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin.The EMBO journal · 1996Article
- Centractin (ARP1) associates with spectrin revealing a potential mechanism to link dynactin to intracellular organelles.The Journal of cell biology · 1996Article
- The Saccharomyces cerevisiae actin-related protein Arp2 is involved in the actin cytoskeleton.The Journal of cell biology · 1996Article
- A novel suppressor of ras1 in fission yeast, byr4, is a dosage-dependent inhibitor of cytokinesis.The Journal of cell biology · 1996Article
- Article
- Sequences, structural models, and cellular localization of the actin-related proteins Arp2 and Arp3 from Acanthamoeba.The Journal of cell biology · 1995Article
- The actin-related protein Act3p of Saccharomyces cerevisiae is located in the nucleus.Molecular biology of the cell · 1995Article
- A novel protein kinase gene ssp1+ is required for alteration of growth polarity and actin localization in fission yeast.The EMBO journal · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Actins are a family of highly conserved proteins that are ubiquitously found among eukaryotic organisms. All actins that have previously been identified, including those of animals, plants, fungi, and protozoa, are 374-376 amino acids long and exhibit at least 70% amino acid sequence identity when compared with one another. We have cloned a gene from the fission yeast Schizosaccharomyces pombe that encodes a distantly related member of the actin protein family, herein referred to as act2. In contrast to all other actins, the derived amino acid sequence reveals that act2 is 427 residues long and exhibits only 35-40% identity to actins, including act1 from Sch. pombe. Comparison to the known x-ray crystallographic structure of rabbit skeletal muscle actin indicates that the ATP and divalent metal ion binding sites are largely conserved in act2, while regions involved in actin-actin and actin-myosin interactions are relatively divergent. Disruption of the act2 gene demonstrated that this gene encodes a function essential for germination of haploid spores. These findings indicate that while act2 and act1 are related proteins, they appear to have distinct functions. In addition, they demonstrate that the actin protein family is more diverse than was previously thought.
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