ArticleMolecular and cellular biology2007
A+U-rich instability elements differentially activate 5'-3' and 3'-5' mRNA decay.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 61 citations in OpenAlex.
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- New insights into decapping enzymes and selective mRNA decay.Wiley interdisciplinary reviews. RNA · 2017Review
- Oligoadenylation of 3' decay intermediates promotes cytoplasmic mRNA degradation in Drosophila cells.RNA (New York, N.Y.) · 2016Article
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- The C-terminal extension of Lsm4 interacts directly with the 3' end of the histone mRNP and is required for efficient histone mRNA degradation.RNA (New York, N.Y.) · 2014Article
- A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stability.RNA (New York, N.Y.) · 2012Article
- Identification of cytoplasmic capping targets reveals a role for cap homeostasis in translation and mRNA stability.Cell reports · 2012Article
- Identification of the human PMR1 mRNA endonuclease as an alternatively processed product of the gene for peroxidasin-like protein.RNA (New York, N.Y.) · 2012Article
- Regulation of cytoplasmic mRNA decay.Nature reviews. Genetics · 2012Review
- Global analysis of the mammalian RNA degradome reveals widespread miRNA-dependent and miRNA-independent endonucleolytic cleavage.Nucleic acids research · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The A+U-rich elements (or AREs) are cis-acting sequences that activate rapid mRNA decay, yet the overall polarity of this process is unknown. The current study describes an unbiased approach to this using the Invader RNA assay (Third Wave Technologies, Inc.) to quantify the decay of each of the three exons of human beta-globin mRNA without added instability elements or with the AREs from c-fos or granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA in the 3' untranslated region. Each of these genes under tetracycline operator control was stably transfected into cells, and beta-globin mRNA was quantified with exon-specific probes following transcription termination. There was little overall evidence for polarity in stable mRNA decay. Adding the c-fos ARE activated rapid and simultaneous decay from both ends of the mRNA. In contrast, the GM-CSF ARE activated decay primarily from the mRNA 5' end. These data were supported by reciprocal RNA interference knockdowns, and we present evidence that the 5'-3' and 3'-5' decay pathways are functionally linked.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.