ArticleProtein expression and purification2007
Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium.
Article in Protein expression and purification, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Autophagy of the somatic stalk cells likely nurses the propagating spores of Dictyostelid social amoebas.Open research Europe · 2022Article
- The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy.Autophagy · 2021Article
- Article
- The Dictyostelium discoideum homologue of Twinkle, Twm1, is a mitochondrial DNA helicase, an active primase and promotes mitochondrial DNA replication.BMC molecular biology · 2018Article
- Cell-type specific RNA-Seq reveals novel roles and regulatory programs for terminally differentiated Dictyostelium cells.BMC genomics · 2018Article
- Glycogen synthase kinase 3 promotes multicellular development over unicellular encystation in encysting Dictyostelia.EvoDevo · 2018Article
- A conserved signalling pathway for amoebozoan encystation that was co-opted for multicellular development.Scientific reports · 2015Article
- Article
- Mef2A, a homologue of animal Mef2 transcription factors, regulates cell differentiation in Dictyostelium discoideum.BMC developmental biology · 2013Article
- A kinesin-mediated mechanism that couples centrosomes to nuclei.Cellular and molecular life sciences : CMLS · 2013Article
- Evolution of self-organisation in Dictyostelia by adaptation of a non-selective phosphodiesterase and a matrix component for regulated cAMP degradation.Development (Cambridge, England) · 2012Article
- Two novel Src homology 2 domain proteins interact to regulate dictyostelium gene expression during growth and early development.The Journal of biological chemistry · 2010Article
- Article
- Activated cAMP receptors switch encystation into sporulation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The carboxy-terminal domain of Dictyostelium C-module-binding factor is an independent gene regulatory entity.PloS one · 2009Article
- From drought sensing to developmental control: evolution of cyclic AMP signaling in social amoebas.Molecular biology and evolution · 2008Article
- A protein expression system for tandem affinity purification in Xanthomonas citri subsp. citri.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We constructed a series of expression vectors for purification of native proteins and protein complexes in Dictyostelium. Protein purification is achieved by either a C-terminal or N-terminal fusion of the protein of choice to the tandem affinity purification (TAP) tag. The TAP tag consists of a protein A tag and a calmodulin binding peptide (CBP) and has been successfully used for purification of native protein complexes from yeast and animal cells. Protein expression is driven by the constitutive actin 15 promoter and the vectors optionally carry additional green- or yellow fluorescent protein (GFP or YFP) tags for fusion at either a C- or N-terminal location. Tandem affinity purification of native Dictyostelium protein complexes was tested by using pArc-34, one of the members of the well characterized Dictyostelium Arp2/3 complex, as bait. After denaturation and SDS-PAGE separation of the pArc-34 associated proteins all members of the Arp2/3 complex could be identified.
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