ArticlePlant molecular biology2007
Promoter analysis and immunolocalisation show that puroindoline genes are exclusively expressed in starchy endosperm cells of wheat grain.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 61 citations in OpenAlex.
- The Role ofInternational journal of molecular sciences · 2025Review
- Over-Expression of a Wheat Late Maturity Alpha-Amylase Type 1 Impact on Starch Properties During Grain Development and Germination.Frontiers in plant science · 2022Article
- Dissecting the Role of Promoters of Pathogen-sensitive Genes in Plant Defense.Current genomics · 2020Review
- Marker-assisted transfer of3 Biotech · 2019Article
- The trafficking pathway of a wheat storage protein in transgenic rice endosperm.Annals of botany · 2014Article
- Expression pattern of the alpha-kafirin promoter coupled with a signal peptide from Sorghum bicolor L. Moench.Journal of biomedicine & biotechnology · 2012Article
- Transgenic rice plants harboring the grain hardness-locus region of Aegilops tauschii.Plant cell reports · 2011Article
- Isolation of the endosperm-specific LPAAT gene promoter from coconut (Cocos nucifera L.) and its functional analysis in transgenic rice plants.Plant cell reports · 2010Article
- Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency.Journal of experimental botany · 2010Article
- The determinants of grain texture in cereals.Journal of applied genetics · 2009Review
- Trafficking of storage proteins in developing grain of wheat.Journal of experimental botany · 2009Article
- Expression of cholera toxin B subunit in transgenic rice endosperm.Molecular biotechnology · 2008Article
- Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development.Annals of botany · 2008Article
- Efficient and rapid Agrobacterium-mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes.Transgenic research · 2008Article
- Expression pattern and activity of six glutelin gene promoters in transgenic rice.Journal of experimental botany · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 4 countries.
Funding
Abstract
The purolindolines are small cysteine-rich proteins which are present in the grain of wheat. They have a major impact on the utilisation of the grain as they are the major determinants of grain texture, which affects both milling and baking properties. Bread and durum wheats were transformed with constructs comprising the promoter regions of the Puroindoline a (Pina) and Puroindoline b (Pinb) genes fused to the uidA (GUS) reporter gene. Nine lines showing 3:1 segregation for the transgene and comprising all transgene/species combinations were selected for detailed analysis of transgene expression during grain development. This showed that transgene expression occurred only in the starchy endosperm cells and was not observed in any other seed or vegetative tissues. The location of the puroindoline proteins in these cells was confirmed by tissue printing of developing grain, using a highly specific monoclonal antibody for detection and an antibody to the aleurone-localised 8S globulin as a control. This provides clear evidence that puroindolines are only synthesised and accumulated in the starchy endosperm cells of the wheat grain.
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