ArticlePlant molecular biology2007
Plastid marker gene excision by the phiC31 phage site-specific recombinase.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 75 citations in OpenAlex.
- Editing and synthetic engineering of chloroplast genomes.Plant communications · 2026Review
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Cyanobacterial photosystem II reaction center design in tobacco chloroplasts increases biomass in low light.Plant physiology · 2023Article
- Plastid Marker Gene Excision in the Tobacco Shoot Apex by Agrobacterium-Delivered Cre Recombinase.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Marker-Free Transplastomic Plants by Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Review
- Small serine recombination systems ParA-MRS and CinH-RS2 perform precise excision of plastid DNA.Plant biotechnology journal · 2017Article
- Construction of Plastid Expression Vector and Development of Genetic Transformation System for the Seaweed Pyropia yezoensis.Marine biotechnology (New York, N.Y.) · 2017Article
- Cell-to-cell movement of mitochondria in plants.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco.International journal of molecular sciences · 2015Article
- Review
- Recent developments in therapeutic protein expression technologies in plants.Biotechnology letters · 2015Review
- Plastid genomics in horticultural species: importance and applications for plant population genetics, evolution, and biotechnology.Frontiers in plant science · 2015Review
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
- Stable plastid transformation for high-level recombinant protein expression: promises and challenges.Journal of biomedicine & biotechnology · 2012Article
- Article
- Visual spectinomycin resistance (aadA(au)) gene for facile identification of transplastomic sectors in tobacco leaves.Plant molecular biology · 2011Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- Study of plastid genome stability in tobacco reveals that the loss of marker genes is more likely by gene conversion than by recombination between 34-bp loxP repeats.Plant physiology · 2010Article
- Transgene excision from wheat chromosomes by phage phiC31 integrase.Plant molecular biology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marker genes are essential for selective amplification of rare transformed plastid genome copies to obtain genetically stable transplastomic plants. However, the marker gene becomes dispensable when homoplastomic plants are obtained. Here we report excision of plastid marker genes by the phiC31 phage site-specific integrase (Int) that mediates recombination between bacterial (attB) and phage (attP) attachment sites. We tested marker gene excision in a two-step process. First we transformed the tobacco plastid genome with the pCK2 vector in which the spectinomycin resistance (aadA) marker gene is flanked with suitably oriented attB and attP sites. The transformed plastid genomes were stable in the absence of Int. We then transformed the nucleus with a gene encoding a plastid-targeted Int that led to efficient marker gene excision. The aadA marker free Nt-pCK2-Int plants were resistant to phosphinothricin herbicides since the pCK2 plastid vector also carried a bar herbicide resistance gene that, due to the choice of its promoter, causes a yellowish-golden (aurea) phenotype. Int-mediated marker excision reported here is an alternative to the currently used CRE/loxP plastid marker excision system and expands the repertoire of the tools available for the manipulation of the plastid genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.