ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2007
Simultaneous real-time PCR detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 68 citations in OpenAlex.
- Review
- Multiplex PCR System for the Diagnosis of Plague.Current molecular medicine · 2025Review
- Vacuum Oven Drying: A Cost-Effective Way of Producing Field-Deployable Reagents for In-house Real-Time PCR Methods.Molecular biotechnology · 2025Article
- Molecular and serological investigation of Francisella tularensis among wild animals in Yamaguchi prefecture.Veterinary research communications · 2024Article
- Wild lagomorphs as potential sources ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Positive Francisella tularensis meningitis outcome despite delayed identification: a case report.Annals of clinical microbiology and antimicrobials · 2023Article
- Applications of polymerase chain reaction-based methods for the diagnosis of plague (Review).Experimental and therapeutic medicine · 2022Review
- Review
- Enhanced threat of tick-borne infections within cities? Assessing public health risks due to ticks in urban green spaces in Helsinki, Finland.Zoonoses and public health · 2020Article
- Duplex Electrochemical DNA Sensor to DetectACS omega · 2019Article
- Tick-borne pathogens in Finland: comparison of Ixodes ricinus and I. persulcatus in sympatric and parapatric areas.Parasites & vectors · 2018Article
- Sensitive Detection of Francisella tularensis Directly from Whole Blood by Use of the GeneXpert System.Journal of clinical microbiology · 2017Article
- Detection of Francisella tularensis in voles in Finland.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2014Article
- Cross-institute evaluations of inhibitor-resistant PCR reagents for direct testing of aerosol and blood samples containing biological warfare agent DNA.Applied and environmental microbiology · 2014Article
- Experimental Infection of voles with Francisella tularensis indicates their amplification role in tularemia outbreaks.PloS one · 2014Article
- Simultaneous and Rapid Detection of Salmonella typhi, Bacillus anthracis, and Yersinia pestis by Using Multiplex Polymerase Chain Reaction (PCR).Iranian Red Crescent medical journal · 2013Article
- Reverse line blot macroarray for simultaneous detection and characterization of four biological warfare agents.Indian journal of microbiology · 2013Article
- Yersinia pestis plasminogen activator gene homolog in rat tissues.Emerging infectious diseases · 2013Article
- Evaluation of inhibitor-resistant real-time PCR methods for diagnostics in clinical and environmental samples.PloS one · 2013Article
- Novel genomic tools for specific and real-time detection of biothreat and frequently encountered foodborne pathogens.Journal of food protection · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This report describes the development of in-house real-time PCR assays using minor groove binding probes for simultaneous detection of the Bacillus anthracis pag and cap genes, the Francisella tularensis 23 KDa gene, as well as the Yersinia pestis pla gene. The sensitivities of these assays were at least 1 fg, except for the assay targeting the Bacillus anthracis cap gene, which showed a sensitivity of 10 fg when total DNA was used as a template in a serial dilution. The clinical value of the Bacillus anthracis- and Francisella tularensis-specific assays was demonstrated by successful amplification of DNA from cases of cow anthrax and hare tularemia, respectively. No cross-reactivity between these species-specific assays or with 39 other bacterial species was noted. These assays may provide a rapid tool for the simultaneous detection and identification of the three category A bacterial species listed as biological threats by the Centers for Disease Control and Prevention.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.