ArticleMolecular and cellular biochemistry2007
Morphology, cytoskeletal organization, and myosin dynamics of mouse embryonic fibroblasts cultured on nanofibrillar surfaces.
Article in Molecular and cellular biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Engineering a multicompartment in vitro model for dorsal root ganglia phenotypic assessment.Journal of biomedical materials research. Part B, Applied biomaterials · 2023Article
- Nano-Structured Ridged Micro-Filaments (≥100 µm Diameter) Produced Using a Single Step Strategy for Improved Bone Cell Adhesion and Proliferation in Textile Scaffolds.Molecules (Basel, Switzerland) · 2022Article
- A low-cost microwell device for high-resolution imaging of neurite outgrowth in 3D.Journal of neural engineering · 2018Article
- Optimization of Polymer-ECM Composite Scaffolds for Tissue Engineering: Effect of Cells and Culture Conditions on Polymeric Nanofiber Mats.Journal of functional biomaterials · 2017Article
- Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall.Journal of visualized experiments : JoVE · 2015Article
- Nanofibers for drug delivery - incorporation and release of model molecules, influence of molecular weight and polymer structure.Beilstein journal of nanotechnology · 2015Article
- Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Decoding information in cell shape.Cell · 2013Article
- Behavior of mouse spermatogonial stem-like cells on an electrospun nanofibrillar matrix.Journal of assisted reproduction and genetics · 2013Article
- Article
- Geometry sensing by dendritic cells dictates spatial organization and PGE(2)-induced dissolution of podosomes.Cellular and molecular life sciences : CMLS · 2012Article
- The regulation of focal adhesion complex formation and salivary gland epithelial cell organization by nanofibrous PLGA scaffolds.Biomaterials · 2012Article
- A short review: Recent advances in electrospinning for bone tissue regeneration.Journal of tissue engineering · 2012Article
- Theoretical model for cellular shapes driven by protrusive and adhesive forces.PLoS computational biology · 2011Article
- Mechano-topographic modulation of stem cell nuclear shape on nanofibrous scaffolds.Acta biomaterialia · 2011Article
- The heel and toe of the cell's foot: a multifaceted approach for understanding the structure and dynamics of focal adhesions.Cell motility and the cytoskeleton · 2009Review
- New directions in nanofibrous scaffolds for soft tissue engineering and regeneration.Expert review of medical devices · 2009Review
- Ovarian normal and tumor-associated fibroblasts retain in vivo stromal characteristics in a 3-D matrix-dependent manner.Gynecologic oncology · 2008Article
- Phase transitions of the coupled membrane-cytoskeleton modify cellular shape.Biophysical journal · 2007Article
- Designing synthetic materials to control stem cell phenotype.Current opinion in chemical biology · 2007Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Growth of cells in tissue culture is generally performed on two-dimensional (2D) surfaces composed of polystyrene or glass. Recent work, however, has shown that such 2D cultures are incomplete and do not adequately represent the physical characteristics of native extracellular matrix (ECM)/basement membrane (BM), namely dimensionality, compliance, fibrillarity, and porosity. In the current study, a three-dimensional (3D) nanofibrillar surface composed of electrospun polyamide nanofibers was utilized to mimic the topology and physical structure of ECM/BM. Additional chemical cues were incorporated into the nanofibrillar matrix by coating the surfaces with fibronectin, collagen I, or laminin-1. Results from the current study show an enhanced response of primary mouse embryonic fibroblasts (MEFs) to culture on nanofibrillar surfaces with more dramatic changes in cell spreading and reorganization of the cytoskeleton than previously observed for established cell lines. In addition, the cells cultured on nanofibrillar and 2D surfaces exhibited differential responses to the specific ECM/BM coatings. The localization and activity of myosin II-B for MEFs cultured on nanofibers was also compared. A dynamic redistribution of myosin II-B was observed within membrane protrusions. This was previously described for cells associated with nanofibers composed of collagen I but not for cells attached to 2D surfaces coated with monomeric collagen. These results provide further evidence that nanofibrillar surfaces offer a significantly different environment for cells than 2D substrates.
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