ArticleJournal of bacteriology2007
Contributions of [4Fe-4S]-FNR and integration host factor to fnr transcriptional regulation.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Functional analysis of the methylerythritol phosphate pathway terminal enzymes IspG and IspH fromMicrobiology spectrum · 2024Article
- Role of themBio · 2023Article
- Small RNA ArcZ Regulates Oxidative Stress Response Genes and Regulons inFrontiers in microbiology · 2019Article
- Identification and Unusual Properties of the Master Regulator FNR in the Extreme AcidophileFrontiers in microbiology · 2019Article
- Reassessing the Structure and Function Relationship of the OAntioxidants & redox signaling · 2018Review
- Design principles of a conditional futile cycle exploited for regulation.Molecular bioSystems · 2015Article
- Fe-S proteins that regulate gene expression.Biochimica et biophysica acta · 2015Review
- DNA looping-dependent autorepression of LEE1 P1 promoters by Ler in enteropathogenic Escherichia coli (EPEC).Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The mononuclear molybdenum enzymes.Chemical reviews · 2014Review
- The influence of promoter architectures and regulatory motifs on gene expression in Escherichia coli.PloS one · 2014Article
- Genome-scale analysis of escherichia coli FNR reveals complex features of transcription factor binding.PLoS genetics · 2013Article
- Regulation of iron-sulphur cluster homeostasis through transcriptional control of the Isc pathway by [2Fe-2S]-IscR in Escherichia coli.Molecular microbiology · 2013Article
- Anaerobic expression of the gadE-mdtEF multidrug efflux operon is primarily regulated by the two-component system ArcBA through antagonizing the H-NS mediated repression.Frontiers in microbiology · 2013Article
- ArcA and AppY antagonize IscR repression of hydrogenase-1 expression under anaerobic conditions, revealing a novel mode of O2 regulation of gene expression in Escherichia coli.Journal of bacteriology · 2012Article
- The multidrug efflux pump MdtEF protects against nitrosative damage during the anaerobic respiration in Escherichia coli.The Journal of biological chemistry · 2011Article
- Phenotypic repertoire of the FNR regulatory network in Escherichia coli.Molecular microbiology · 2011Article
- Repression of aerobic leukotoxin transcription by integration host factor in Aggregatibacter actinomycetemcomitans.Research in microbiology · 2010Article
- Regulation of aerobic-to-anaerobic transitions by the FNR cycle in Escherichia coli.Journal of molecular biology · 2010Article
- The O2 sensitivity of the transcription factor FNR is controlled by Ser24 modulating the kinetics of [4Fe-4S] to [2Fe-2S] conversion.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Redox control of the DNA damage-inducible protein DinG helicase activity via its iron-sulfur cluster.The Journal of biological chemistry · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Maintaining appropriate levels of the global regulator FNR is critical to its function as an O(2) sensor. In this study, we examined the mechanisms that control transcription of fnr to increase our understanding of how FNR protein levels are regulated. Under anaerobic conditions, one mechanism that controls fnr expression is negative autoregulation by the active [4Fe-4S] form of FNR. Through DNase I footprinting and in vitro transcription experiments, we observed that direct binding of [4Fe-4S]-FNR to the predicted downstream FNR binding site is sufficient for repression of the fnr promoter in vitro. In addition, the downstream FNR binding site was required for repression of transcription from fnr'-lacZ fusions in vivo. No repression of fnr was observed in vivo or in vitro with the apoprotein form of FNR, indicating that repression requires the dimeric, Fe-S cluster-containing protein. Furthermore, our in vitro and in vivo data suggest that [4Fe-4S]-FNR does not bind to the predicted upstream FNR binding site within the fnr promoter. Rather, we provide evidence that integration host factor binds to this upstream region and increases in vivo expression of Pfnr under both aerobic and anaerobic conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.