ArticleBiophysical journal2007
Second harmonic and sum frequency generation imaging of fibrous astroglial filaments in ex vivo spinal tissues.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 93 citations in OpenAlex.
- Second harmonic generation for brain imaging: pathology-related studies.Biophysical reviews · 2025Article
- Commercially derived versatile optical architecture for two-photon STED, wavelength mixing and label-free microscopy.Biomedical optics express · 2022Article
- Applications of second harmonic generation (SHG)/sum-frequency generation (SFG) imaging for biophysical characterization of the plasma membrane.Biophysical reviews · 2020Review
- Spatially dependent H-bond dynamics at interfaces of water/biomimetic self-assembled lattice materials.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Label-free Optical Imaging of Membrane Potential.Current opinion in biomedical engineering · 2019Article
- Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy.Scientific reports · 2017Article
- New advances in probing cell-extracellular matrix interactions.Integrative biology : quantitative biosciences from nano to macro · 2017Review
- Advances in Intravital Non-Linear Optical Imaging of the Central Nervous System in Rodents.Brain plasticity (Amsterdam, Netherlands) · 2016Review
- Measuring microtubule polarity in spindles with second-harmonic generation.Biophysical journal · 2014Article
- Theoretical and experimental SHG angular intensity patterns from healthy and proteolysed muscles.Biophysical journal · 2013Article
- Modeling of supramolecular centrosymmetry effect on sarcomeric SHG intensity pattern of skeletal muscles.Biophysical journal · 2011Article
- Multimodal Nonlinear Optical Microscopy.Laser & photonics reviews · 2011Article
- Real-time CARS imaging reveals a calpain-dependent pathway for paranodal myelin retraction during high-frequency stimulation.PloS one · 2011Article
- Stent-induced coronary artery stenosis characterized by multimodal nonlinear optical microscopy.Journal of biomedical optics · 2011Article
- Imaging of zebrafish in vivo with second-harmonic generation reveals shortened sarcomeres associated with myopathy induced by statin.PloS one · 2011Article
- Shedding new light on lipid biology with coherent anti-Stokes Raman scattering microscopy.Journal of lipid research · 2010Review
- Imaging growth of neurites in conditioned hydrogel by coherent anti-stokes raman scattering microscopy.Organogenesis · 2009Article
- Imaging and quantitative analysis of atherosclerotic lesions by CARS-based multimodal nonlinear optical microscopy.Arteriosclerosis, thrombosis, and vascular biology · 2009Article
- Article
- Chasing lipids in health and diseases by coherent anti-Stokes Raman scattering microscopy.Vibrational Spectroscopy · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Sum frequency generation (SFG) and second harmonic generation (SHG) were observed from helical fibrils in spinal cord white matter isolated from guinea pigs. By combining SFG with coherent anti-Stokes Raman scattering microscopy, which allows visualization of myelinated axons, these fibers were found to be distributed near the surface of the spinal cord, between adjacent axons, and along the blood vessels. Using 20-microm-thick tissue slices, the ratio of forward to backward SHG signal from large bundles was found to be much larger than that from small single fibrils, indicating a phase-matching effect in coherent microscopy. Based on the intensity profiles across fibrils and the size dependence of forward and backward signal from the same fibril, we concluded that the main SHG signal directly originates from the fibrils, but not from surface SHG effects. Further polarization analysis of the SHG signal showed that the symmetry property of the fibril could be well described with a cylindrical model. Colocalization of the SHG signal with two-photon excitation fluorescence (TPEF) from the immunostaining of glial fibrillary acidic protein demonstrated that SHG arises from astroglial filaments. This assignment was further supported by colocalization of the SHG contrast with TPEF signals from astrocyte processes labeled by a Ca(2+) indicator and sulforhodamine 101. This work shows that a combination of three nonlinear optical imaging techniques--coherent anti-Stokes Raman scattering, TPEF, and SHG (SFG) microscopy--allows simultaneous visualization of different structures in a complex biological system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.