ArticleBiophysical journal2007
Multiple association states between glycine receptors and gephyrin identified by SPT analysis.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Glycine receptor and release site organization impacts the kinetics of glycinergic synapse currents.Biophysical journal · 2025Article
- Chronic benzodiazepine treatment triggers gephyrin scaffold destabilization and GABAFrontiers in cellular neuroscience · 2025Article
- APP and Bace1: Differential effect of cholesterol enrichment on processing and plasma membrane mobility.iScience · 2023Article
- Reciprocal stabilization of glycine receptors and gephyrin scaffold proteins at inhibitory synapses.Biophysical journal · 2021Article
- Light Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapses.Frontiers in synaptic neuroscience · 2021Review
- Linking Nanoscale Dynamics of AMPA Receptor Organization to Plasticity of Excitatory Synapses and Learning.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Review
- A Simple and Powerful Analysis of Lateral Subdiffusion Using Single Particle Tracking.Biophysical journal · 2017Article
- GABANature communications · 2017Article
- Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers.Scientific reports · 2017Article
- In Vivo Measurement of Glycine Receptor Turnover and Synaptic Size Reveals Differences between Functional Classes of Motoneurons in Zebrafish.Current biology : CB · 2017Article
- An aggregation-removal model for the formation and size determination of post-synaptic scaffold domains.PLoS computational biology · 2017Article
- Molecular insights into cell toxicity of a novel familial amyloidogenic variant of β2-microglobulin.Journal of cellular and molecular medicine · 2016Article
- Automatic detection of diffusion modes within biological membranes using back-propagation neural network.BMC bioinformatics · 2016Article
- Defects of the Glycinergic Synapse in Zebrafish.Frontiers in molecular neuroscience · 2016Review
- Radixin regulates synaptic GABAA receptor density and is essential for reversal learning and short-term memory.Nature communications · 2015Article
- Membrane distribution of the glycine receptor α3 studied by optical super-resolution microscopy.Histochemistry and cell biology · 2014Article
- Gephyrin clusters are absent from small diameter primary afferent terminals despite the presence of GABA(A) receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.Nature communications · 2014Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The scaffolding protein gephyrin is known to anchor glycine receptors (GlyR) at synapses and to participate in the dynamic equilibrium between synaptic and extrasynaptic GlyR in the neuronal membrane. Here we investigated the properties of this interaction in cells cotransfected with YFP-tagged gephyrin and GlyR subunits possessing an extracellular myc-tag. In HeLa cells and young neurons, single particle tracking was used to follow in real time individual GlyR, labeled with quantum dots, traveling into and out of gephyrin clusters. Analysis of the diffusion properties of two GlyR subunit types--able or unable to bind gephyrin--gave access to the association states of GlyR with its scaffolding protein. Our results indicated that an important portion of GlyR could be linked to a few molecules of gephyrin outside gephyrin clusters. This emphasizes the role of scaffolding proteins in the extrasynaptic membrane and supports the implication of gephyrin-gephyrin interactions in the stabilization of GlyR at synapses. The kinetic parameters controlling the equilibrium between GlyR inside and outside clusters were also characterized. Within clusters, we identified two subpopulations of GlyR with distinct degrees of stabilization between receptors and scaffolding proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.