ArticleInfection and immunity1992
Shigella flexneri enters human colonic Caco-2 epithelial cells through the basolateral pole.
Article in Infection and immunity, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 131 papers.
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Who cites it
131 citing papers in PubMed.
- O-specific [corrected] polysaccharide conjugate vaccine-induced [corrected] antibodies prevent invasion of Shigella into Caco-2 cells and may be curative.Proceedings of the National Academy of Sciences of the United States of America · 2007Trial
- Sequential invasion of epithelial and immune cells in Shigellosis.bioRxiv : the preprint server for biology · 2026Article
- High pathogenic potential of Shigella flexneri strains descending from a common ancestor isolated over 34 years in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Determinants of divergentmBio · 2025Article
- A three-dimensional high throughput assay identifies novel antibacterial molecules with activity against intracellular Shigella.npj antimicrobials and resistance · 2025Article
- Engagement of αFrontiers in microbiology · 2024Article
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- Pathogenicity and virulence ofVirulence · 2023Review
- Uptake ofInternational journal of molecular sciences · 2023Article
- Functional assays to evaluate antibody-mediated responses againstFrontiers in cellular and infection microbiology · 2023Review
- 4D live imaging and computational modeling of a functional gut-on-a-chip evaluate how peristalsis facilitates enteric pathogen invasion.Science advances · 2022Article
- SepA EnhancesmBio · 2021Article
- Shigella flexneri Diguanylate Cyclases Regulate Virulence.Journal of bacteriology · 2021Article
- Refining Host-Pathogen Interactions: Organ-on-Chip Side of the Coin.Pathogens (Basel, Switzerland) · 2021Review
- Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.PloS one · 2021Article
- Research in a time of enteroids and organoids: how the human gut model has transformed the study of enteric bacterial pathogens.Gut microbes · 2020Review
- Article
- Transcytosis subversion by M cell-to-enterocyte spread promotes Shigella flexneri and Listeria monocytogenes intracellular bacterial dissemination.PLoS pathogens · 2020Article
- Functional antibodies as immunological endpoints to evaluate protective immunity againstHuman vaccines & immunotherapeutics · 2020Review
- Article
71 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The commonly accepted view that enteroinvasive bacteria enter cells of the intestinal epithelial lining through the apical surface can be challenged in the case of shigellosis. This study is based on in vitro experiments that showed that the invasion of human colonic Caco-2 cells by Shigella flexneri occurred through the basolateral pole of these cells. In these experiments, the few bacteria that interacted with the apical surface either bound to microvilli of the cell dome without causing detectable alteration or bound at the level of intercellular junctions at which they demonstrated a limited capacity for paracellular invasion, which permitted subsequent entry through the lateral domain of the cells. Treatment of Caco-2 cell monolayers with ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), which disrupts intercellular junctions, greatly enhanced the rate of cell infection. These observations suggest a physiopathological paradox that may have important consequences for the understanding of the process of colonic invasion in vivo during shigellosis.
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Registered trials
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