ArticleJournal of controlled release : official journal of the Controlled Release Society2007
Ultrasonically targeted delivery into endothelial and smooth muscle cells in ex vivo arteries.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- Flow rate modulates focused ultrasound-mediated vascular delivery of microRNA.Molecular therapy. Nucleic acids · 2025Article
- Effect of Photo-Mediated Ultrasound Therapy on Nitric Oxide and Prostacyclin from Endothelial Cells.Applied sciences (Basel, Switzerland) · 2022Article
- Ultrasound-Targeted Microbubble Destruction: Modulation in the Tumor Microenvironment and Application in Tumor Immunotherapy.Frontiers in immunology · 2022Review
- The effect of ultrasound cavitation on endothelial cells.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Reducing Neointima Formation in a Swine Model with IVUS and Sirolimus Microbubbles.Annals of biomedical engineering · 2015Article
- High Efficiency Molecular Delivery with Sequential Low-Energy Sonoporation Bursts.Theranostics · 2015Article
- Effect of ultrasound on the permeability of vascular wall to nano-emulsion droplets.Ultrasound in medicine & biology · 2013Article
- Quantitative evaluation of ultrasound-mediated cellular uptake of a fluorescent model drug.Molecular imaging and biology · 2013Article
- Clot retraction affects the extent of ultrasound-enhanced thrombolysis in an ex vivo porcine thrombosis model.Ultrasound in medicine & biology · 2013Article
- Real-time assessment of ultrasound-mediated drug delivery using fibered confocal fluorescence microscopy.Molecular imaging and biology · 2013Article
- Acoustic radiation force for vascular cell therapy: in vitro validation.Ultrasound in medicine & biology · 2012Article
- Effects of ultrasound and ultrasound contrast agent on vascular tissue.Cardiovascular ultrasound · 2012Article
- Can ultrasound enable efficient intracellular uptake of molecules? A retrospective literature review and analysis.Ultrasound in medicine & biology · 2012Review
- Phase-shift, stimuli-responsive drug carriers for targeted delivery.Therapeutic delivery · 2011Review
- Real-time technique for improving molecular imaging and guiding drug delivery in large blood vessels: in vitro and ex vivo results.Molecular imaging · 2011Article
- Evaluation of the temporal window for drug delivery following ultrasound-mediated membrane permeability enhancement.Molecular imaging and biology · 2011Article
- Spatiotemporal effects of sonoporation measured by real-time calcium imaging.Ultrasound in medicine & biology · 2009Article
- Microfabricated implants for applications in therapeutic delivery, tissue engineering, and biosensing.Lab on a chip · 2008Review
- Microbubbles in ultrasound-triggered drug and gene delivery.Advanced drug delivery reviews · 2008Review
- Mechanistic analysis of chemical permeation enhancers for oral drug delivery.Pharmaceutical research · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
This study tested the hypothesis that ultrasound can target intracellular uptake of drugs into vascular endothelial cells (ECs) at low to intermediate energy and into smooth muscle cells (SMCs) at high energy. Ultrasound-enhanced delivery has been shown to enhance and target intracellular drug and gene delivery in the vasculature to treat cardiovascular disease, but quantitative studies of the delivery process are lacking. Viable ex vivo porcine carotid arteries were placed in a solution containing a model drug, TO-PRO(R)-1, and Optison microbubbles. Arteries were exposed to ultrasound at 1.1 MHz and acoustic energies of 5.0, 66, or 630 J/cm(2). Using confocal microscopy and fluorescent labeling of cells, the artery endothelium and media were imaged to determine the localization and to quantify intracellular uptake and cell death. At low to intermediate ultrasound energy, ultrasound was shown to target intracellular delivery into viable cells that represented 9-24% of exposed ECs. These conditions also typically caused 7-25% EC death. At high energy, intracellular delivery was targeted to SMCs, which was associated with denuding or death of proximal ECs. This work represents the first known in-depth study to evaluate intracellular uptake into cells in tissue. We conclude that significant intracellular uptake of molecules can be targeted into ECs and SMCs by ultrasound-enhanced delivery suggesting possible applications for treatment of cardiovascular diseases and dysfunctions.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.