ArticleGenes & development2007
Cdk phosphorylation of the Ste11 transcription factor constrains differentiation-specific transcription to G1.
Article in Genes & development, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Constitutively active RAS prolongs Cdc42 signalling, while MAPK signalling is attenuated during fission yeast mating.PLoS genetics · 2026Article
- Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.PLoS biology · 2024Article
- Cyclin-dependent kinases: Masters of the eukaryotic universe.Wiley interdisciplinary reviews. RNA · 2023Review
- The ecl family gene ecl3+ is induced by phosphate starvation and contributes to sexual differentiation in fission yeast.Journal of cell science · 2023Article
- Sporulation: A response to starvation in the fission yeast Schizosaccharomyces pombe.MicrobiologyOpen · 2022Review
- Cell cycle-dependent and independent mating blocks ensure fungal zygote survival and ploidy maintenance.PLoS biology · 2021Article
- Requirement of PP2A-B56iScience · 2020Article
- Computational modelling of meiotic entry and commitment.Scientific reports · 2018Article
- Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.Journal of cell science · 2017Article
- The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe.PloS one · 2012Article
- Neurospora crassa fmf-1 encodes the homologue of the Schizosaccharomyces pombe Ste11p regulator of sexual development.Journal of genetics · 2009Article
- The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8.Genes & development · 2008Article
- Counteractive control of polarized morphogenesis during mating by mitogen-activated protein kinase Fus3 and G1 cyclin-dependent kinase.Molecular biology of the cell · 2008Article
- Cdc2p controls the forkhead transcription factor Fkh2p by phosphorylation during sexual differentiation in fission yeast.The EMBO journal · 2008Article
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Authors and funding
4 authors.
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Abstract
Eukaryotic cells normally differentiate from G(1); here we investigate the mechanism preventing expression of differentiation-specific genes outside G(1). In fission yeast, induction of the transcription factor Ste11 triggers sexual differentiation. We find that Ste11 is only active in G(1) when Cdk activity is low. In the remaining part of the cell cycle, Ste11 becomes Cdk-phosphorylated at Thr 82 (T82), which inhibits its DNA-binding activity. Since the ste11 gene is autoregulated and the Ste11 protein is highly unstable, this Cdk switch rapidly extinguishes Ste11 activity when cells enter S phase. When we mutated T82 to aspartic acid, mimicking constant phosphorylation, cells no longer underwent differentiation. Conversely, changing T82 to alanine rendered Ste11-controlled transcription constitutive through the cell cycle, and allowed mating from S phase with increased frequency. Thus, Cdk phosphorylation mediates periodic expression of Ste11 and its target genes, and we suggest this to be part of the mechanism restricting differentiation to G(1).
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