ArticleNucleic acids research2007
Quantitative and mechanism-based investigation of post-nuclear delivery events between adenovirus and lipoplex.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 111 citations in OpenAlex.
- mRNA and Adenoviral Vector Vaccine Platforms Utilized in COVID-19 Vaccines: Technologies, Ecosystem, and Future Directions.Vaccines · 2023Review
- Oncolytic virus: A catalyst for the treatment of gastric cancer.Frontiers in oncology · 2022Review
- Understanding In Vivo Fate of Nucleic Acid and Gene Medicines for the Rational Design of Drugs.Pharmaceutics · 2021Review
- Current Status and Challenges Associated with CNS-Targeted Gene Delivery across the BBB.Pharmaceutics · 2020Review
- Role of Cellular Retention and Intracellular State in Controlling Gene Delivery Efficiency of Multiple Nonviral Carriers.ACS omega · 2019Article
- Low Electric Treatment activates Rho GTPase via Heat Shock Protein 90 and Protein Kinase C for Intracellular Delivery of siRNA.Scientific reports · 2019Article
- Glucocorticoid Cell Priming Enhances Transfection Outcomes in Adult Human Mesenchymal Stem Cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- The novel functional nucleic acid iRed effectively regulates target genes following cytoplasmic delivery by faint electric treatment.Science and technology of advanced materials · 2016Article
- Mechanistic evaluation of the transfection barriers involved in lipid-mediated gene delivery: interplay between nanostructure and composition.Biochimica et biophysica acta · 2014Article
- Article
- Nucleic acid delivery: the missing pieces of the puzzle?Accounts of chemical research · 2012Review
- The statistics of protein expression ratios for cellular fluorescence studies.European biophysics journal : EBJ · 2012Article
- Intracellular trafficking of polyamidoamine-poly(ethylene glycol) block copolymers in DNA delivery.Bioconjugate chemistry · 2011Article
- DNA nuclear targeting sequences for non-viral gene delivery.Pharmaceutical research · 2011Article
- Quantitative analysis of condensation/decondensation status of pDNA in the nuclear sub-domains by QD-FRET.Nucleic acids research · 2011Article
- Emerging links between surface nanotechnology and endocytosis: impact on nonviral gene delivery.Nano today · 2010Article
- Intracellular trafficking and decondensation kinetics of chitosan-pDNA polyplexes.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Particle tracking of intracellular trafficking of octaarginine-modified liposomes: a comparative study with adenovirus.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Lipid-based systemic delivery of siRNA.Advanced drug delivery reviews · 2009Review
- Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative and mechanism-based information on differences in transfection efficiency between viral and non-viral vectors would be highly useful for improving the effectiveness of non-viral vectors. A previous quantitative comparison of intracellular trafficking between adenovirus and LipofectAMINE PLUS (LFN) revealed that the three orders of magnitude lower transfection efficiency of LFN was dominantly rate limited by the post-nuclear delivery process. In the present study, the contribution of transcription and translation processes to the overall differences in the transgene expression efficiency of nucleus-delivered DNA was independently evaluated by quantifying mRNA. As a result, transcription efficiency (E(transcript)) of LFN, denoted as transgene expression divided by the amount of nuclear pDNA was about 16 times less than that for adenovirus. Furthermore, translation efficiency (E(translate)), denoted as transfection activity divided by mRNA expression was approximately 460 times less in LFN. Imaging of the decondensed form of DNA by in situ hybridization revealed that poor decondensation efficiency of LFN is involved in the inferior E(transcript). Moreover, the inferior translation efficiency (E(translate)) of LFN was mainly due to electrostatic interactions between LFN and mRNA. Collectively, an improvement in nuclear decondensation and the diminution of the interaction between vector and mRNA is essential for the development of new generations of non-viral vectors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.