ArticleNucleic acids research2007
Small ncRNA transcriptome analysis from kinetoplast mitochondria of Leishmania tarentolae.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Advances in Protozoan Epigenetic Targets and Their Inhibitors for the Development of New Potential Drugs.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Current Status of Regulatory Non-Coding RNAs Research in the Tritryp.Non-coding RNA · 2022Review
- MitosRNAs and extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus.Scientific reports · 2019Article
- Review
- RNA binding and core complexes constitute the U-insertion/deletion editosome.Molecular and cellular biology · 2014Article
- Review
- Organelle trafficking of chimeric ribozymes and genetic manipulation of mitochondria.Nucleic acids research · 2011Article
- Mitochondrial RNA processing in trypanosomes.Research in microbiology · 2011Review
- cDNA library generation from ribonucleoprotein particles.Nature protocols · 2011Article
- RNPomics: defining the ncRNA transcriptome by cDNA library generation from ribonucleo-protein particles.Nucleic acids research · 2010Article
- RET1-catalyzed uridylylation shapes the mitochondrial transcriptome in Trypanosoma brucei.Molecular and cellular biology · 2010Article
- Methodological obstacles in knocking down small noncoding RNAs.RNA (New York, N.Y.) · 2009Article
- Roles for TbDSS-1 in RNA surveillance and decay of maturation by-products from the 12S rRNA locus.Nucleic acids research · 2008Article
- Uridine insertion/deletion editing in trypanosomes: a playground for RNA-guided information transfer.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene expression in mitochondria of kinetoplastid protozoa requires RNA editing, a post-transcriptional process which involves insertion or deletion of uridine residues at specific sites within mitochondrial pre-mRNAs. Sequence specificity of the RNA editing process is mediated by oligo-uridylated small, non-coding RNAs, designated as guide RNAs (gRNAs). In this study, we have analyzed the small ncRNA transcriptome from kinetoplast mitochondria of Leishmania tarentolae by generating specialized cDNA libraries encoding size-selected RNA species. Through this screen, a significant number of novel oligo-uridylated RNA species, which we have termed oU-RNAs, has been identified. Most novel oU-RNAs are present as stable RNA species in mitochondria as assessed by northern blot analysis. Thereby, novel oU-RNAs show similar expression levels and sizes as previously reported for canonical gRNAs. Several oU-RNAs are transcribed from both strands of the maxicircle and minicircles components of the mitochondrial genome, from regions where up till now no transcription has been reported. Two stable oU-RNAs exhibit an anchor sequence in antisense orientation to known gRNAs and thus might regulate editing of respective pre-mRNAs. A number of oU-RNAs map in antisense orientation to non-edited protein-coding genes suggesting that they might function by a different mechanism. In addition, our screen shows that all kinetoplast-derived RNAs are prone to some degree of uridylation.
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