ArticleCell motility and the cytoskeleton2007
The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons.
Article in Cell motility and the cytoskeleton, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- Long-term prognostic value of longitudinal measurements of blood neurofilament levels.Neurology(R) neuroimmunology & neuroinflammation · 2020Trial
- Exclusion of CLIC5 as a Candidate Gene and Identification of NEFM as a Possible Novel Gene Correlated With Autosomal Recessive Pure Cerebellar Ataxia in a Highly Consanguineous Family.Molecular genetics & genomic medicine · 2026Article
- Age-dependent remodelling of arterial chemoafferent innervation in hypertension.Cardiovascular research · 2025Article
- Trends in clinical studies evaluating neurofilament light chain as a biomarker.Biomarkers in medicine · 2025Article
- Progress toward a comprehensive brain protein interactome.Biochemical Society transactions · 2025Review
- Neurofilament Biophysics: From Structure to Biomechanics.Molecular biology of the cell · 2024Review
- Biofluid biomarkers for Alzheimer's disease.Frontiers in aging neuroscience · 2024Review
- Minimal genetically encoded tags for fluorescent protein labeling in living neurons.Nature communications · 2022Article
- Plasma Neurofilament Light Chain as a Translational Biomarker of Aging and Neurodegeneration in Dogs.Molecular neurobiology · 2020Article
- Charcot-Marie-Tooth disease Type 2E/1F mutant neurofilament proteins assemble into neurofilaments.Cytoskeleton (Hoboken, N.J.) · 2019Article
- Cerebrospinal fluid biomarkers for understanding multiple aspects of Alzheimer's disease pathogenesis.Cellular and molecular life sciences : CMLS · 2019Review
- Paraneoplastic neuronal intermediate filament autoimmunity.Neurology · 2018Article
- Kymograph analysis with high temporal resolution reveals new features of neurofilament transport kinetics.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Sternopygus macrurus electric organ transcriptome and cell size exhibit insensitivity to short-term electrical inactivity.Journal of physiology, Paris · 2016Article
- Live-cell imaging of neurofilament transport in cultured neurons.Methods in cell biology · 2016Article
- Intrinsically disordered proteins drive membrane curvature.Nature communications · 2015Article
- Severing and end-to-end annealing of neurofilaments in neurons.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Neurofilaments at a glance.Journal of cell science · 2012Review
- Neurofilaments are flexible polymers that often fold and unfold, but they move in a fully extended configuration.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Peripherin is a subunit of peripheral nerve neurofilaments: implications for differential vulnerability of CNS and peripheral nervous system axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Studies on the axonal transport of neurofilament proteins in cultured neurons have shown they move at fast rates, but their overall rate of movement is slow because they spend most of their time not moving. Using correlative light and electron microscopy, we have shown that these proteins move in the form of assembled neurofilament polymers. However, the polypeptide composition of these moving polymers is not known. To address this, we visualized neurofilaments in cultured neonatal mouse sympathetic neurons using GFP-tagged neurofilament protein M and performed time-lapse fluorescence microscopy of naturally occurring gaps in the axonal neurofilament array. When neurofilaments entered the gaps, we stopped them in their tracks using a rapid perfusion and permeabilization technique and then processed them for immunofluorescence microscopy. To compare moving neurofilaments to the total neurofilament population, most of which are stationary at any point in time, we also performed immunofluorescence microscopy on neurofilaments in detergent-splayed axonal cytoskeletons. All neurofilaments, both moving and stationary, contained NFL, NFM, peripherin and alpha-internexin along>85% of their length. NFH was absent due to low expression levels in these neonatal neurons. These data indicate that peripherin and alpha-internexin are integral and abundant components of neurofilament polymers in these neurons and that both moving and stationary neurofilaments in these neurons are complex heteropolymers of at least four different neuronal intermediate filament proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.