ArticlePflugers Archiv : European journal of physiology2007
Regulation of membrane excitability by intracellular pH (pHi) changers through Ca2+-activated K+ current (BK channel) in single smooth muscle cells from rabbit basilar artery.
Article in Pflugers Archiv : European journal of physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Potassium channels in vascular smooth muscle cells.Physiological reviews · 2026Review
- Regulation of Autonomic Motility in Human Gastric Muscle and the Gastroepiploic Artery by Stretch and pH.Journal of muscle research and cell motility · 2025Article
- Metabolic Regulatory Mechanism of Gastric Motility by Acetylcholine.Gastroenterology research · 2025Article
- Cell Settling, Migration, and Stochastic Cancer Gene Expression Suggest Potassium Membrane Flux May Initiate pH Reversal.Biomolecules · 2025Article
- The Large-Conductance, Calcium-Activated Potassium Channel: A Big Key Regulator of Cell Physiology.Frontiers in physiology · 2021Review
- Role of Airway Smooth Muscle in Inflammation Related to Asthma and COPD.Advances in experimental medicine and biology · 2021Article
- Calcium- and voltage-gated BK channels in vascular smooth muscle.Pflugers Archiv : European journal of physiology · 2018Review
- Acid-base regulation and sensing: Accelerators and brakes in metabolic regulation of cerebrovascular tone.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2018Review
- Smooth Muscle Ion Channels and Regulation of Vascular Tone in Resistance Arteries and Arterioles.Comprehensive Physiology · 2017Review
- Myometrial relaxation of mice via expression of two pore domain acid sensitive K(+) (TASK-2) channels.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2016Article
- H2 Receptor-Mediated Relaxation of Circular Smooth Muscle in Human Gastric Corpus: the Role of Nitric Oxide (NO).The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2014Article
- Mechanism of Relaxation Via TASK-2 Channels in Uterine Circular Muscle of Mouse.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2013Article
- Function and regulation of large conductance Ca(2+)-activated K+ channel in vascular smooth muscle cells.Drug discovery today · 2012Review
- Molecular and electrophysiological characterization of a novel cation channel of Trypanosoma cruzi.PLoS pathogens · 2012Article
- The emerging chondrocyte channelome.Frontiers in physiology · 2010Article
- Modulation of BKCa channel gating by endogenous signaling molecules.Physiology (Bethesda, Md.) · 2009Review
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Employing microfluorometric system and patch clamp technique in rabbit basilar arterial myocytes, regulation mechanisms of vascular excitability were investigated by applying intracellular pH (pH(i)) changers such as sodium acetate (SA) and NH(4)Cl. Applications of caffeine produced transient phasic contractions in a reversible manner. These caffeine-induced contractions were significantly enhanced by SA and suppressed by NH(4)Cl. Intracellular Ca(2+) concentration ([Ca(2+)](i)) was monitored in a single isolated myocyte and based the ratio of fluorescence using Fura-2 AM (R (340/380)). SA (20 mM) increased and NH(4)Cl (20 mM) decreased R (340/380) by 0.2 +/- 0.03 and 0.1 +/- 0.02, respectively, in a reversible manner. Caffeine (10 mM) transiently increased R (340/380) by 0.9 +/- 0.07, and the ratio increment was significantly enhanced by SA and suppressed by NH(4)Cl, implying that SA and NH(4)Cl may affect [Ca(2+)](i) (p < 0.05). Accordingly, we studied the effects of SA and NH(4)Cl on Ca(2+)-activated K(+) current (IK(Ca)) under patch clamp technique. Caffeine produced transient outward current at holding potential (V (h)) of 0 mV, caffeine induced transient outward K(+) current, and the spontaneous transient outward currents were significantly enhanced by SA and suppressed by NH(4)Cl. In addition, IK(Ca) was significantly increased by acidotic condition when pH(i) was lowered by altering the NH(4)Cl gradient across the cell membrane. Finally, the effects of SA and NH(4)Cl on the membrane excitability and basal tension were studied: Under current clamp mode, resting membrane potential (RMP) was -28 +/- 2.3 mV in a single cell level and was depolarized by 13 +/- 2.4 mV with 2 mM tetraethylammonium (TEA). SA hyperpolarized and NH(4)Cl depolarized RMP by 10 +/- 1.9 and 16 +/- 4.7 mV, respectively. SA-induced hyperpolarization and relaxation of basal tension was significantly inhibited by TEA. These results suggest that SA and NH(4)Cl might regulate vascular tone by altering membrane excitability through modulation of [Ca(2+)](i) and Ca(2+)-activated K channels in rabbit basilar artery.
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