ArticleNucleic acids research2007
Translational repression of mouse mu opioid receptor expression via leaky scanning.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Epigenetic Modulation of Opioid Receptors by Drugs of Abuse.International journal of molecular sciences · 2022Review
- The role of upstream open reading frames in translation regulation in the apicomplexan parasitesParasitology · 2021Review
- Src-dependent phosphorylation of μ-opioid receptor at TyrEMBO molecular medicine · 2017Article
- Post-Transcriptional Regulation of the Human Mu-Opioid Receptor (MOR) by Morphine-Induced RNA Binding Proteins hnRNP K and PCBP1.Journal of cellular physiology · 2017Article
- Article
- Vimentin interacts with the 5'-untranslated region of mouse mu opioid receptor (MOR) and is required for post-transcriptional regulation.RNA biology · 2013Article
- Post-transcriptional regulation of mu-opioid receptor: role of the RNA-binding proteins heterogeneous nuclear ribonucleoprotein H1 and F.Cellular and molecular life sciences : CMLS · 2012Article
- p38 mitogen-activated protein kinase and PI3-kinase are involved in up-regulation of mu opioid receptor transcription induced by cycloheximide.Journal of neurochemistry · 2011Article
- Ultraconserved cDNA segments in the human transcriptome exhibit resistance to folding and implicate function in translation and alternative splicing.Nucleic acids research · 2011Article
- Transcriptional and epigenetic regulation of opioid receptor genes: present and future.Annual review of pharmacology and toxicology · 2011Review
- Up-regulation of the mu-opioid receptor gene is mediated through chromatin remodeling and transcriptional factors in differentiated neuronal cells.Molecular pharmacology · 2010Article
- uAUG-mediated translational initiations are responsible for human mu opioid receptor gene expression.Journal of cellular and molecular medicine · 2010Article
- Unified translation repression mechanism for microRNAs and upstream AUGs.BMC genomics · 2010Article
- The 5'-untranslated region of multidrug resistance associated protein 2 (MRP2; ABCC2) regulates downstream open reading frame expression through translational regulation.Molecular pharmacology · 2010Article
- Epigenetic programming of mu-opioid receptor gene in mouse brain is regulated by MeCP2 and Brg1 chromatin remodelling factor.Journal of cellular and molecular medicine · 2009Article
- Differential use of an in-frame translation initiation codon regulates human mu opioid receptor (OPRM1).Cellular and molecular life sciences : CMLS · 2009Article
- Evolution of alternative and constitutive regions of mammalian 5'UTRs.BMC genomics · 2009Article
- Long-term morphine treatment decreases the association of mu-opioid receptor (MOR1) mRNA with polysomes through miRNA23b.Molecular pharmacology · 2009Article
- Post-transcriptional regulation of mouse mu opioid receptor (MOR1) via its 3' untranslated region: a role for microRNA23b.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- A proteomics approach for identification of single strand DNA-binding proteins involved in transcriptional regulation of mouse mu opioid receptor gene.Molecular & cellular proteomics : MCP · 2008Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Mu opioid receptor (MOR) expression is under temporal and spatial controls, but expression levels of the MOR gene are relatively low in vivo. In addition to transcriptional regulations, upstream AUGs (uAUGs) and open reading frames (uORFs) profoundly affect the translation of the primary ORF and thus the protein levels in several genes. The 5'-untranslated region (UTR) of mouse MOR mRNA contains three uORFs preceding the MOR main initiation codon. In MOR-fused EGFP or MOR promoter/luciferase reporter constructs, mutating each uAUG individually or in combinations increased MOR transient heterologous expression in neuroblastoma NMB and HEK293 cells significantly. Translation of such constructs increased up to 3-fold without altering the mRNA levels if either the third uAUG or both the second and third AUGs were mutated. Additionally, these uAUG-mediated translational inhibitions were independent of their peptide as confirmed by internal mutation analyses in each uORF. Translational studies indicated that protein syntheses were initiated at these uAUG initiation sites, with the third uAUG initiating the highest translation level. These results support the hypothesis that uORFs in mouse MOR mRNA act as negative regulators through a ribosome leaky scanning mechanism. Such leaky scanning resulted in the suppression of mouse MOR under normal conditions.
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