ArticleThe Journal of cell biology2007
Residual active granzyme B in cathepsin C-null lymphocytes is sufficient for perforin-dependent target cell apoptosis.
Article in The Journal of cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 65 citations in OpenAlex.
- Granzyme B and melittin in cancer immunotherapy: molecular mechanisms and therapeutic perspectives in head and neck cancers.Frontiers in immunology · 2025Review
- Pharmacologic inhibition of dipeptidyl peptidase 1 (cathepsin C) does not blockFrontiers in pharmacology · 2024Article
- Lysosomal peptidases-intriguing roles in cancer progression and neurodegeneration.FEBS open bio · 2022Review
- Granzymes: The Molecular Executors of Immune-Mediated Cytotoxicity.International journal of molecular sciences · 2022Review
- Human CD4+ T-Cell Clone Expansion Leads to the Expression of the Cysteine Peptidase Inhibitor Cystatin F.International journal of molecular sciences · 2021Article
- Extracellular Cystatin F Is Internalised by Cytotoxic T Lymphocytes and Decreases Their Cytotoxicity.Cancers · 2020Article
- Cysteine Cathepsins in Tumor-Associated Immune Cells.Frontiers in immunology · 2019Review
- Cystatin F as a regulator of immune cell cytotoxicity.Cancer immunology, immunotherapy : CII · 2018Review
- Glucosamine prevents polarization of cytotoxic granules in NK-92 cells by disturbing FOXO1/ERK/paxillin phosphorylation.PloS one · 2018Article
- Regulation of perforin activation and pre-synaptic toxicity through C-terminal glycosylation.EMBO reports · 2017Article
- Cystatin F Affects Natural Killer Cell Cytotoxicity.Frontiers in immunology · 2017Article
- Protease signaling in animal and plant-regulated cell death.The FEBS journal · 2016Review
- Regulation of split anergy in natural killer cells by inhibition of cathepsins C and H and cystatin F.Oncotarget · 2015Article
- A natural genetic variant of granzyme B confers lethality to a common viral infection.PLoS pathogens · 2014Article
- A colorimetric assay that specifically measures Granzyme B proteolytic activity: hydrolysis of Boc-Ala-Ala-Asp-S-Bzl.Journal of visualized experiments : JoVE · 2014Article
- Cysteine cathepsins as regulators of the cytotoxicity of NK and T cells.Frontiers in immunology · 2014Review
- Article
- Cathepsin C limits acute viral infection independently of NK cell and CD8+ T-cell cytolytic function.Immunology and cell biology · 2011Article
- Human and mouse perforin are processed in part through cleavage by the lysosomal cysteine proteinase cathepsin L.Immunology · 2010Article
- Cathepsin H is an additional convertase of pro-granzyme B.The Journal of biological chemistry · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cathepsin C activates serine proteases expressed in hematopoietic cells by cleaving an N-terminal dipeptide from the proenzyme upon granule packaging. The lymphocytes of cathepsin C-null mice are therefore proposed to totally lack granzyme B activity and perforin-dependent cytotoxicity. Surprisingly, we show, using live cell microscopy and other methodologies, that cells targeted by allogenic CD8(+) cytotoxic T lymphocyte (CTL) raised in cathepsin C-null mice die through perforin-dependent apoptosis indistinguishable from that induced by wild-type CTL. The cathepsin C-null CTL expressed reduced but still appreciable granzyme B activity, but minimal granzyme A activity. Also, in contrast to mice with inactivation of both their granzyme A/B genes, cathepsin C deficiency did not confer susceptibility to ectromelia virus infection in vivo. Overall, our results indicate that although cathepsin C clearly generates the majority of granzyme B activity, some is still generated in its absence, pointing to alternative mechanisms for granzyme B processing and activation. Cathepsin C deficiency also results in considerably milder immune deficiency than perforin or granzyme A/B deficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.