ArticleApplied and environmental microbiology2007
Prevalence of Shiga toxin-producing Escherichia coli stx1, stx2, eaeA, and rfbE genes and survival of E. coli O157:H7 in manure from organic and low-input conventional dairy farms.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 89 citations in OpenAlex.
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- Effect of Turning Frequency on the Survival of Fecal Indicator Microorganisms during Aerobic Composting of Fecal Sludge with Sawdust.International journal of environmental research and public health · 2023Article
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- Interaction between Fungal Communities, Soil Properties, and the Survival of InvadingInternational journal of environmental research and public health · 2020Article
- Investigation of the Causes of ShigatoxigenicMicroorganisms · 2020Article
- Prevalence and concentration of stx+ E. coli and E. coli O157 in bovine manure from Florida farms.PloS one · 2019Article
- Shiga (Vero)-toxin producingAntimicrobial resistance and infection control · 2017Article
- Prevalence of sorbitol non-fermenting Shiga toxin-producing Escherichia coli in Black Bengal goats on smallholdings.Epidemiology and infection · 2016Article
- Dynamics of Escherichia coli Virulence Factors in Dairy Herds and Farm Environments in a Longitudinal Study in the United States.Applied and environmental microbiology · 2015Article
- Distribution and Characterization of Salmonella enterica Isolates from Irrigation Ponds in the Southeastern United States.Applied and environmental microbiology · 2015Article
- Sorbitol non-fermenting shiga toxin-producing Escherichia coli in cattle on smallholdings.Epidemiology and infection · 2015Article
- Agricultural and management practices and bacterial contamination in greenhouse versus open field lettuce production.International journal of environmental research and public health · 2014Article
- Enteric pathogen survival varies substantially in irrigation water from Belgian lettuce producers.International journal of environmental research and public health · 2014Article
- Isolation, prevalence, and risk factors for infection by shiga toxin-producing Escherichia coli (STEC) in dairy cattle.Tropical animal health and production · 2014Article
- Exserohilum rostratum: characterization of a cross-kingdom pathogen of plants and humans.PloS one · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Manure samples were collected from 16 organic (ORG) and 9 low-input conventional (LIC) Dutch dairy farms during August and September 2004 to determine the prevalence of the STEC virulence genes stx(1) (encoding Shiga toxin 1), stx(2) (encoding Shiga toxin 2), and eaeA (encoding intimin), as well as the rfbE gene, which is specific for Escherichia coli O157. The rfbE gene was present at 52% of the farms. The prevalence of rfbE was higher at ORG farms (61%) than at LIC farms (36%), but this was not significant. Relatively more LIC farms were positive for all Shiga toxin-producing E. coli (STEC) virulence genes eaeA, stx(1), and stx(2), which form a potentially highly virulent combination. Species richness of Enterobacteriaceae, as determined by DGGE, was significantly lower in manure positive for rfbE. Survival of a green fluorescent protein-expressing E. coli O157:H7 strain was studied in the manure from all farms from which samples were obtained and was modeled by a biphasic decline model. The time needed to reach the detection limit was predominantly determined by the level of native coliforms and the pH (both negative relationships). Initial decline was faster for ORG manure but leveled off earlier, resulting in longer survival than in LIC manure. Although the nonlinear decline curve could theoretically be explained as the cumulative distribution of an underlying distribution of decline kinetics, it is proposed that the observed nonlinear biphasic pattern of the survival curve is the result of changing nutrient status of the manure over time (and thereby changing competition pressure), instead of the presence of subpopulations differing in the level of resistance.
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