ArticleBiophysical journal2007
Dynamics of synaptic SfiI-DNA complex: single-molecule fluorescence analysis.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Assembly of Synaptic Protein-DNA Complexes: Critical Role of Non-Specific Interactions.International journal of molecular sciences · 2023Article
- Direct Detection of α-Synuclein Dimerization Dynamics: Single-Molecule Fluorescence Analysis.Biophysical journal · 2015Article
- Single molecule imaging approach to membrane protein stoichiometry.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2012Review
- Visual analysis of concerted cleavage by type IIF restriction enzyme SfiI in subsecond time region.Biophysical journal · 2011Article
- Effect of single-strand break on branch migration and folding dynamics of Holliday junctions.Biophysical journal · 2010Article
- AFM for analysis of structure and dynamics of DNA and protein-DNA complexes.Methods (San Diego, Calif.) · 2009Article
- Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence.Biophysical journal · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A single-molecule analysis was applied to study the dynamics of synaptic and presynaptic DNA-protein complexes (binding of two DNA and one DNA duplex, respectively). In the approach used in this study, the protein was tethered to a surface, allowing a freely diffusing fluorescently labeled DNA to bind to the protein, thus forming a presynaptic complex. The duration of fluorescence burst is the measure of the characteristic lifetime of the complex. To study the formation of the synaptic complex, the two SfiI-bound duplexes with the labeled donor and acceptor were used. The synaptic complex formation by these duplexes was detected by the fluorescence resonance energy transfer approach. The duration of the fluorescence resonance energy transfer burst is the measure of the characteristic lifetime of the synaptic complex. We showed that both synaptic and presynaptic complexes have characteristic dissociation times in the range of milliseconds, with the synaptic SfiI-DNA complex having the shorter dissociation time. Comparison of the off-rate data for the synaptic complex with the rate of DNA cleavage led to the hypothesis that the complex is very dynamic, so the formation of an enzymatically active synaptic complex is a rather rare event in these series of conformational transitions.
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