ArticleJournal of bacteriology2007
Initial steps of colicin E1 import across the outer membrane of Escherichia coli.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- A historical perspective on the multifunctional outer membrane channel protein TolC in Escherichia coli.npj antimicrobials and resistance · 2025Review
- Structural shifts in TolC facilitate Efflux-Mediated β-lactam resistance.Communications biology · 2024Article
- Rapid LC-MS assay for targeted metabolite quantification by serial injection into isocratic gradients.Analytical and bioanalytical chemistry · 2023Article
- Microbiota dynamics, metabolic and immune interactions in the cervicovaginal environment and their role in spontaneous preterm birth.Frontiers in immunology · 2023Review
- Colicin E1 opens its hinge to plug TolC.eLife · 2022Article
- The Antibiotic Efflux Protein TolC Is a Highly Evolvable Target under Colicin E1 or TLS Phage Selection.Molecular biology and evolution · 2021Article
- Tools and Approaches for Dissecting Protein Bacteriocin Import in Gram-Negative Bacteria.Frontiers in microbiology · 2019Review
- The Colicin E1 TolC Box: Identification of a Domain Required for Colicin E1 Cytotoxicity and TolC Binding.Journal of bacteriology · 2017Article
- The Colicin E1 TolC-Binding Conformer: Pillar or Pore Function of TolC in Colicin Import?Biochemistry · 2016Article
- In vivo processing of DNase colicins E2 and E7 is required for their import into the cytoplasm of target cells.PloS one · 2014Article
- FtsH-dependent processing of RNase colicins D and E3 means that only the cytotoxic domains are imported into the cytoplasm.The Journal of biological chemistry · 2011Article
- Precise manipulation of chromosomes in vivo enables genome-wide codon replacement.Science (New York, N.Y.) · 2011Article
- Legionella pneumophila LbtU acts as a novel, TonB-independent receptor for the legiobactin siderophore.Journal of bacteriology · 2011Article
- AcrA suppressor alterations reverse the drug hypersensitivity phenotype of a TolC mutant by inducing TolC aperture opening.Molecular microbiology · 2010Article
- The colicin Ia receptor, Cir, is also the translocator for colicin Ia.Molecular microbiology · 2010Article
- Folding and trimerization of signal sequence-less mature TolC in the cytoplasm of Escherichia coli.Microbiology (Reading, England) · 2009Article
- Assembly and transport mechanism of tripartite drug efflux systems.Biochimica et biophysica acta · 2009Review
- Analysis of YfgL and YaeT interactions through bioinformatics, mutagenesis, and biochemistry.Journal of bacteriology · 2008Article
- Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons of Escherichia coli.Nucleic acids research · 2008Article
- Colicin biology.Microbiology and molecular biology reviews : MMBR · 2007Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Data suggest a two-receptor model for colicin E1 (ColE1) translocation across the outer membrane of Escherichia coli. ColE1 initially binds to the vitamin B(12) receptor BtuB and then translocates through the TolC channel-tunnel, presumably in a mostly unfolded state. Here, we studied the early events in the import of ColE1. Using in vivo approaches, we show that ColE1 is cleaved when added to whole cells. This cleavage requires the presence of the receptor BtuB and the protease OmpT, but not that of TolC. Strains expressing OmpT cleaved ColE1 at K84 and K95 in the N-terminal translocation domain, leading to the removal of the TolQA box, which is essential for ColE1's cytotoxicity. Supported by additional in vivo data, this suggests that a function of OmpT is to degrade colicin at the cell surface and thus protect sensitive E. coli cells from infection by E colicins. A genetic strategy for isolating tolC mutations that confer resistance to ColE1, without affecting other TolC functions, is also described. We provide further in vivo evidence of the multistep interaction between TolC and ColE1 by using cross-linking followed by copurification via histidine-tagged TolC. First, secondary binding of ColE1 to TolC is dependent on primary binding to BtuB. Second, alterations to a residue in the TolC channel interfere with the translocation of ColE1 across the TolC pore rather than with the binding of ColE1 to TolC. In contrast, a substitution at a residue exposed on the cell surface abolishes both binding and translocation of ColE1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.