ArticleAmerican journal of physiology. Lung cellular and molecular physiology2007
A Ba2+-resistant, acid-sensitive K+ conductance in Na+-absorbing H441 human airway epithelial cells.
Article in American journal of physiology. Lung cellular and molecular physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Involvement of butyrate in electrogenic KPflugers Archiv : European journal of physiology · 2019Article
- P2Y receptor regulation of K2P channels that facilitate KAmerican journal of physiology. Cell physiology · 2018Article
- Article
- A role for two-pore potassium (K2P) channels in endometrial epithelial function.Journal of cellular and molecular medicine · 2013Article
- The gasotransmitter hydrogen sulphide decreases Na⁺ transport across pulmonary epithelial cells.British journal of pharmacology · 2012Article
- A role for two-pore K⁺ channels in modulating Na⁺ absorption and Cl⁻ secretion in normal human bronchial epithelial cells.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- External Ba2+ block of the two-pore domain potassium channel TREK-1 defines conformational transition in its selectivity filter.The Journal of biological chemistry · 2011Article
- Dexamethasone-induced up-regulation of two-pore domain K+ channel genes, TASK-1 and TWIK-2, in cultured human periodontal ligament fibroblasts.In vitro cellular & developmental biology. Animal · 2011Article
- K+ channel openers restore verapamil-inhibited lung fluid resolution and transepithelial ion transport.Respiratory research · 2010Article
- Cation currents in human airway epithelial cells induced by infection with influenza A virus.The Journal of physiology · 2009Article
- KCNQ-encoded channels regulate Na+ transport across H441 lung epithelial cells.Pflugers Archiv : European journal of physiology · 2009Article
- Impact of mechanical stress on ion transport in native lung epithelium (Xenopus laevis): short-term activation of Na+, Cl (-) and K+ channels.Pflugers Archiv : European journal of physiology · 2008Article
- Molecular regulations governing TREK and TRAAK channel functions.Channels (Austin, Tex.)Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
By analysis of whole cell membrane currents in Na(+)-absorbing H441 human airway epithelial cells, we have identified a K(+) conductance (G(K)) resistant to Ba(2+) but sensitive to bupivacaine or extracellular acidification. In polarized H441 monolayers, we have demonstrated that bupivacaine, lidocaine, and quinidine inhibit basolateral membrane K(+) current (I(Bl)) whereas Ba(2+) has only a weak inhibitory effect. I(Bl) was also inhibited by basolateral acidification, and, although subsequent addition of bupivacaine caused a further fall in I(Bl), acidification had no effect after bupivacaine, demonstrating that cells grown under these conditions express at least two different bupivacaine-sensitive K(+) channels, only one of which is acid sensitive. Basolateral acidification also inhibited short-circuit current (I(SC)), and basolateral bupivacaine, lidocaine, quinidine, and Ba(2+) inhibited I(SC) at concentrations similar to those needed to inhibit I(Bl), suggesting that the K(+) channels underlying I(Bl) are part of the absorptive mechanism. Analyses using RT-PCR showed that mRNA encoding several two-pore domain K(+) (K2P) channels was detected in cells grown under standard conditions (TWIK-1, TREK-1, TASK-2, TWIK-2, KCNK-7, TASK-3, TREK-2, THIK-1, and TALK-2). We therefore suggest that K2P channels underlie G(K) in unstimulated cells and so maintain the driving force for Na(+) absorption. Since this ion transport process is vital to lung function, K2P channels thus play an important but previously undocumented role in pulmonary physiology.
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