ArticleCurrent biology : CB2007
A role for Cdc2- and PP2A-mediated regulation of Emi2 in the maintenance of CSF arrest.
Article in Current biology : CB, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 51 citations in OpenAlex.
- Emi2 Is Essential for Mouse Spermatogenesis.Cell reports · 2017Article
- Vertebrate Reproduction.Cold Spring Harbor perspectives in biology · 2015Review
- Cyclin B3 is a mitotic cyclin that promotes the metaphase-anaphase transition.Current biology : CB · 2015Article
- The zinc-binding region (ZBR) fragment of Emi2 can inhibit APC/C by targeting its association with the coactivator Cdc20 and UBE2C-mediated ubiquitylation.FEBS open bio · 2014Article
- α-endosulfine (ENSA) regulates exit from prophase I arrest in mouse oocytes.Cell cycle (Georgetown, Tex.) · 2014Article
- Modulation of cell cycle control during oocyte-to-embryo transitions.The EMBO journal · 2013Review
- Molecular changes during egg activation.Current topics in developmental biology · 2013Review
- Punctuated cyclin synthesis drives early embryonic cell cycle oscillations.Molecular biology of the cell · 2012Article
- Regulation of the action of early mitotic inhibitor 1 on the anaphase-promoting complex/cyclosome by cyclin-dependent kinases.The Journal of biological chemistry · 2011Article
- Dimethyl sulfoxide inhibits spontaneous oocyte fragmentation and delays inactivation of maturation promoting factor (MPF) during the prolonged culture of ovulated murine oocytes in vitro.Cytotechnology · 2011Article
- Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1.EMBO reports · 2011Article
- Essential role of protein phosphatase 2A in metaphase II arrest and activation of mouse eggs shown by okadaic acid, dominant negative protein phosphatase 2A, and FTY720.The Journal of biological chemistry · 2011Article
- Regulation of mitochondrial morphology by APC/CCdh1-mediated control of Drp1 stability.Molecular biology of the cell · 2011Article
- Mos in the oocyte: how to use MAPK independently of growth factors and transcription to control meiotic divisions.Journal of signal transduction · 2011Article
- Female infertility in PDE3A(-/-) mice: polo-like kinase 1 (Plk1) may be a target of protein kinase A (PKA) and involved in meiotic arrest of oocytes from PDE3A(-/-) mice.Cell cycle (Georgetown, Tex.) · 2010Article
- Mouse Emi2 as a distinctive regulatory hub in second meiotic metaphase.Development (Cambridge, England) · 2010Article
- Emi2-mediated inhibition of E2-substrate ubiquitin transfer by the anaphase-promoting complex/cyclosome through a D-box-independent mechanism.Molecular biology of the cell · 2010Article
- Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail.Molecular biology of the cell · 2010Article
- Features of programmed cell death in intact Xenopus oocytes and early embryos revealed by near-infrared fluorescence and real-time monitoring.Cell death and differentiation · 2010Article
- The M phase kinase Greatwall (Gwl) promotes inactivation of PP2A/B55delta, a phosphatase directed against CDK phosphosites.Molecular biology of the cell · 2009Article
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundVertebrate oocytes are arrested in metaphase II of meiosis prior to fertilization by cytostatic factor (CSF). CSF enforces a cell-cycle arrest by inhibiting the anaphase-promoting complex (APC), an E3 ubiquitin ligase that targets Cyclin B for degradation. Although Cyclin B synthesis is ongoing during CSF arrest, constant Cyclin B levels are maintained. To achieve this, oocytes allow continuous slow Cyclin B degradation, without eliminating the bulk of Cyclin B, which would induce release from CSF arrest. However, the mechanism that controls this continuous degradation is not understood.
resultsWe report here the molecular details of a negative feedback loop wherein Cyclin B promotes its own destruction through Cdc2/Cyclin B-mediated phosphorylation and inhibition of the APC inhibitor Emi2. Emi2 bound to the core APC, and this binding was disrupted by Cdc2/Cyclin B, without affecting Emi2 protein stability. Cdc2-mediated phosphorylation of Emi2 was antagonized by PP2A, which could bind to Emi2 and promote Emi2-APC interactions.
conclusionsConstant Cyclin B levels are maintained during a CSF arrest through the regulation of Emi2 activity. A balance between Cdc2 and PP2A controls Emi2 phosphorylation, which in turn controls the ability of Emi2 to bind to and inhibit the APC. This balance allows proper maintenance of Cyclin B levels and Cdc2 kinase activity during CSF arrest.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.