ArticleJournal of molecular biology2007
TFB1 or TFB2 is sufficient for Thermococcus kodakaraensis viability and for basal transcription in vitro.
Article in Journal of molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 74 citations in OpenAlex.
- Removal and bypass of restriction modification systems increases transformation efficiency ofFrontiers in microbiology · 2026Article
- The Thermococcales as a model system: historical perspectives and emerging tools.Journal of bacteriology · 2025Review
- Archaeal histone-based chromatin structures regulate transcription elongation rates.Communications biology · 2024Article
- Archaea as a Model System for Molecular Biology and Biotechnology.Biomolecules · 2023Review
- 'All About' Extremophiles.Faculty reviews · 2023Review
- The Hyperthermophilic Restriction-Modification Systems ofFrontiers in microbiology · 2021Article
- Thermococcus kodakarensis provides a versatile hyperthermophilic archaeal platform for protein expression.Methods in enzymology · 2021Article
- Archaeal transcription.Transcription · 2020Review
- Article
- FttA is a CPSF73 homologue that terminates transcription in Archaea.Nature microbiology · 2020Article
- Archaeosine Modification of Archaeal tRNA: Role in Structural Stabilization.Journal of bacteriology · 2020Article
- An overview of 25 years of research on Thermococcus kodakarensis, a genetically versatile model organism for archaeal research.Folia microbiologica · 2020Review
- The Role of Archaeal Chromatin in Transcription.Journal of molecular biology · 2019Review
- Distinct Physiological Roles of the Three Ferredoxins Encoded in the Hyperthermophilic ArchaeonmBio · 2019Article
- A linear pathway for mevalonate production supports growth of Thermococcus kodakarensis.Extremophiles : life under extreme conditions · 2019Article
- TFS and Spt4/5 accelerate transcription through archaeal histone-based chromatin.Molecular microbiology · 2019Article
- A transcriptional factor B paralog functions as an activator to DNA damage-responsive expression in archaea.Nucleic acids research · 2018Article
- Factor-dependent archaeal transcription termination.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The GAN Exonuclease or the Flap Endonuclease Fen1 and RNase HII Are Necessary for Viability of Thermococcus kodakarensis.Journal of bacteriology · 2017Article
- Same same but different: The evolution of TBP in archaea and their eukaryotic offspring.Transcription · 2017Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
Archaeal RNA polymerases (RNAPs) are most similar to eukaryotic RNAP II (Pol II) but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. However, many archaeal genomes encode more than one TFB and/or TBP leading to the hypothesis that different TFB/TBP combinations may be employed to direct initiation from different promoters in Archaea. As a first test of this hypothesis, we have determined the ability of RNAP purified from Thermococcus kodakaraensis (T.k.) to initiate transcription from a variety of T.k. promoters in vitro when provided with T.k. TBP and either TFB1 or TFB2, the two TFBs encoded in the T.k. genome. With every promoter active in vitro, transcription initiation occurred with either TFB1 or TFB2 although the optimum salt concentration for initiation was generally higher for TFB2 (approximately 250 mM K(+)) than for TFB1 (approximately 200 mM K(+)). Consistent with this functional redundancy in vitro, T.k. strains have been constructed with the TFB1- (tfb1; TK1280) or TFB2- (tfb2; TK2287) encoding gene deleted. These mutants exhibit no detectable growth defects under laboratory conditions. Domain swapping between TFB1 and TFB2 has identified a central region that contributes to the salt sensitivity of TFB activity, and deleting residues predicted to form the tip of the B-finger region of TFB2 had no detectable effects on promoter recognition or transcription initiation but did eliminate the production of very short (< or =5 nt) abortive transcripts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.