Evidence map›Paper›PMID 17273915›Full record

ArticleTransgenic research2007

pORE: a modular binary vector series suited for both monocot and dicot plant transformation.

Catherine Coutu, James Brandle, Dan Brown, Kirk Brown, Brian Miki, John Simmonds, Dwayne D Hegedus

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Transgenic research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.

0numbers the graph read from it
0cells of the map it votes in
84citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

84 citing papers in PubMed.

  1. Article
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  4. Article
  5. Plant terminators: the unsung heroes of gene expression.Journal of experimental botany · 2023
    Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Transformation of European Ash (Plants (Basel, Switzerland) · 2021
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Generation of Transgenic Self-IncompatiblePlants (Basel, Switzerland) · 2019
    Article
  19. Article
  20. Article

24 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Catherine CoutuAgriculture and Agrifood Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2.
James Brandle
Dan Brown
Kirk Brown
Brian Miki
John Simmonds
Dwayne D Hegedus

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a series of 14 binary vectors suitable for Agrobacterium-mediated transformation of dicotyledonous plants and adaptable for biolistic transformation of monocotyledonous plants. The vector size has been minimized by eliminating all non-essential elements from the vector backbone and T-DNA regions while maintaining the ability to replicate independently. The smallest of the vector series is 6.3 kb and possesses an extensive multiple cloning site with 21 unique restriction endonuclease sites that are compatible with common cloning, protein expression, yeast two-hybrid and other binary vectors. The T-DNA region was engineered using a synthetic designer oligonucleotide resulting in an entirely modular system whereby any vector element can be independently exchanged. The high copy number ColE1 origin of replication has been included to enhance plasmid yield in Escherichia coli. FRT recombination sites flank the selectable marker cassette regions and allow for in planta excision by FLP recombinase. The pORE series consists of three basic types; an 'open' set for general plant transformation, a 'reporter' set for promoter analysis and an 'expression' set for constitutive expression of transgenes. The sets comprise various combinations of promoters (P (HPL), P (ENTCUP2) and P (TAPADH)), selectable markers (nptII and pat) and reporter genes (gusA and smgfp).

Indexed as

Genetic VectorsPlants, Genetically ModifiedTransformation, GeneticAgrobacterium tumefaciensArabidopsisBrassica napusGlycine maxMolecular Sequence DataPisum sativumPlasmidsZea mays

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.