ArticleCurrent genetics2007
Testing the efficacy of RNA interference constructs in Aspergillus fumigatus.
Article in Current genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 39 citations in OpenAlex.
- A population genomic characterization of copy number variation in the opportunistic fungal pathogen Aspergillus fumigatus.PloS one · 2018Article
- Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus.Eukaryotic cell · 2015Article
- Upgrading fungal gene expression on demand: improved systems for doxycycline-dependent silencing in Aspergillus fumigatus.Applied and environmental microbiology · 2013Article
- Altered trichothecene biosynthesis in TRI6-silenced transformants of Fusarium culmorum influences the severity of crown and foot rot on durum wheat seedlings.Molecular plant pathology · 2011Article
- RNA-mediated gene silencing in the cereal fungal pathogen Cochliobolus sativus.Molecular plant pathology · 2011Article
- RNA interference pathways in filamentous fungi.Cellular and molecular life sciences : CMLS · 2010Review
- In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA.BMC biology · 2010Article
- pHg/pSILBAγ vector system for efficient gene silencing in homobasidiomycetes: optimization of ihpRNA - triggering in the mycorrhizal fungus Laccaria bicolor.Microbial biotechnology · 2010Article
- Efflux-mediated antifungal drug resistance.Clinical microbiology reviews · 2009Review
- Discovery of a small-molecule inhibitor of {beta}-1,6-glucan synthesis.Antimicrobial agents and chemotherapy · 2009Article
- Silencing of mitochondrial alternative oxidase gene of Aspergillus fumigatus enhances reactive oxygen species production and killing of the fungus by macrophages.Journal of bioenergetics and biomembranes · 2008Article
- Efficient cloning system for construction of gene silencing vectors in Aspergillus niger.Applied microbiology and biotechnology · 2008Article
- Genome-wide fitness test and mechanism-of-action studies of inhibitory compounds in Candida albicans.PLoS pathogens · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We recently developed a silencing vector in Aspergillus fumigatus which carries a hygromycin resistance marker and a transcriptional unit for hairpin RNA expression under the control of the inducible glucoamylase promoter (pGla) (Mouyna et al. in FEMS Microbiol Lett 237:317-324, 2004). We showed previously that this vector can be used for the RNA interference application of two genes ALB1 and FKS1 of which reduced mRNA levels occurred for both, with phenotypic consequences resembling disruptions of genes involved in melanin (ALB1) and beta(1-3)glucan biosynthesis (FKS1). We reported here the silencing of KRE6 and CRH1, two other genes putatively involved in cell wall biosynthesis using a similar construction under the control of the constitutive promoter glyceraldehyde-3-phosphate dehydrogenase (pgpdA). Silencing of the expression of these two genes was obtained. Further analysis of the transformants showed however that (1) a 100% loss of expression was never achieved for all genes tested (2) the vector used for RNAi is lost or modified over successive transfers resulting in an inhibition of the silencing. These disadvantages of RNAi indicate that classical gene disruption by gene replacement remains the most efficient method for a molecular analysis of gene function in A. fumigatus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.