ArticleJournal of proteome research2007
Profiling the membrane proteome of Shewanella oneidensis MR-1 with new affinity labeling probes.
Article in Journal of proteome research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- Article
- Genomic characterization of rare earth binding by Shewanella oneidensis.Scientific reports · 2023Article
- Single molecule tracking of bacterial cell surface cytochromes reveals dynamics that impact long-distance electron transport.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- In-Cell Labeling and Mass Spectrometry for Systems-Level Structural Biology.Chemical reviews · 2022Review
- C-terminal processing of GlyGly-CTERM containing proteins by rhombosortase in Vibrio cholerae.PLoS pathogens · 2018Article
- Characterization of ExeM, an Extracellular Nuclease ofFrontiers in microbiology · 2018Article
- In vivo protein interaction network analysis reveals porin-localized antibiotic inactivation in Acinetobacter baumannii strain AB5075.Nature communications · 2016Article
- Ion Mobility-Mass Spectrometry Analysis of Cross-Linked Intact Multiprotein Complexes: Enhanced Gas-Phase Stabilities and Altered Dissociation Pathways.Analytical chemistry · 2016Article
- Host-Microbe Protein Interactions during Bacterial Infection.Chemistry & biology · 2015Article
- Probing the protein interaction network of Pseudomonas aeruginosa cells by chemical cross-linking mass spectrometry.Structure (London, England : 1993) · 2015Article
- Solid-phase extraction and purification of membrane proteins using a UV-modified PMMA microfluidic bioaffinity μSPE device.The Analyst · 2014Article
- Protein interactions, post-translational modifications and topologies in human cells.Molecular & cellular proteomics : MCP · 2013Article
- Functional specificity of extracellular nucleases of Shewanella oneidensis MR-1.Applied and environmental microbiology · 2012Article
- Review
- Molecular Underpinnings of Fe(III) Oxide Reduction by Shewanella Oneidensis MR-1.Frontiers in microbiology · 2012Article
- Cross-linking measurements of in vivo protein complex topologies.Molecular & cellular proteomics : MCP · 2011Article
- Roles of two Shewanella oneidensis MR-1 extracellular endonucleases.Applied and environmental microbiology · 2011Article
- A new cross-linking strategy: protein interaction reporter (PIR) technology for protein-protein interaction studies.Molecular bioSystems · 2010Review
- Quantitative analysis of cell surface membrane proteins using membrane-impermeable chemical probe coupled with 18O labeling.Journal of proteome research · 2010Article
- A photocleavable and mass spectrometry identifiable cross-linker for protein interaction studies.Analytical chemistry · 2010Article
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Authors and funding
6 authors.
Funding
Abstract
The membrane proteome plays a critical role in electron transport processes in Shewanella oneidensis MR-1, a bacterial organism that has great potential for bioremediation. Biotinylation of intact cells with subsequent affinity-enrichment has become a useful tool for characterization of the membrane proteome. As opposed to these commonly used, water-soluble commercial reagents, we here introduce a family of hydrophobic, cell-permeable affinity probes for extensive labeling and detection of membrane proteins. When applied to S. oneidensis cells, all three new chemical probes allowed identification of a substantial proportion of membrane proteins from total cell lysate without the use of specific membrane isolation method. From a total of 410 unique proteins identified, approximately 42% are cell envelope proteins that include outer membrane, periplasmic, and inner membrane proteins. This report demonstrates the first application of this intact cell biotinylation method to S. oneidensis and presents the results of many identified proteins that are involved in metal reduction processes. As a general labeling method, all chemical probes we introduced in this study can be extended to other organisms or cell types and will help expedite the characterization of membrane proteomes.
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