ArticleBiotechnology progress
Photolithographic patterning of C2C12 myotubes using vitronectin as growth substrate in serum-free medium.
Article in Biotechnology progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 69 citations in OpenAlex.
- Effect of transforming growth factor beta receptor I inhibitors on myotube formation in vitro.Scientific reports · 2025Article
- Engineering large-scale hiPSC-derived vessel-integrated muscle-like lattices for enhanced volumetric muscle regeneration.Trends in biotechnology · 2024Article
- Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance.International journal of molecular sciences · 2024Article
- Mimicking the Tendon Microenvironment to Enhance Skeletal Muscle Adhesion and Longevity in a Functional Microcantilever Platform.ACS biomaterials science & engineering · 2023Article
- The ensured proliferative capacity of myoblast in serum-reduced conditions with Methyl-β-cyclodextrin.Frontiers in cell and developmental biology · 2023Article
- Serum-free cultures of C2C12 cells show different muscle phenotypes which can be estimated by metabolic profiling.Scientific reports · 2022Article
- Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Electrically Conductive Micropatterned Polyaniline-Poly(ethylene glycol) Composite Hydrogel.Materials (Basel, Switzerland) · 2021Article
- New volumetric CNT-doped gelatin-cellulose scaffolds for skeletal muscle tissue engineering.Nanoscale advances · 2020Article
- Controlled Arrangement of Neuronal Cells on Surfaces Functionalized with Micropatterned Polymer Brushes.ACS omega · 2018Article
- Self-contained, low-cost Body-on-a-Chip systems for drug development.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Vitronectin as a Micromanager of Cell Response in Material-Driven Fibronectin Nanonetworks.Advanced biosystems · 2017Article
- Muscular dystrophy in a dish: engineered human skeletal muscle mimetics for disease modeling and drug discovery.Drug discovery today · 2016Review
- Microfabricated mammalian organ systems and their integration into models of whole animals and humans.Lab on a chip · 2013Review
- A topographically modified substrate-embedded MEA for directed myotube formation at electrode contact sites.Annals of biomedical engineering · 2013Article
- Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels.PloS one · 2013Article
- Patterned cardiomyocytes on microelectrode arrays as a functional, high information content drug screening platform.Biomaterials · 2011Article
- Growth and electrophysiological properties of rat embryonic cardiomyocytes on hydroxyl- and carboxyl-modified surfaces.Journal of biomaterials science. Polymer edition · 2008Article
- Skeletal myotube integration with planar microelectrode arrays in vitro for spatially selective recording and stimulation: a comparison of neuronal and myotube extracellular action potentials.Biotechnology progressArticle
- Differentiation and sarcomere formation in skeletal myocytes directly prepared from human induced pluripotent stem cells using a sphere-based culture.Differentiation; research in biological diversityArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The C2C12 cell line is frequently used as a model of skeletal muscle differentiation. In our serum-free defined culture system, differentiation of C2C12 cells into myotubes required surface-bound signals such as substrate-adsorbed vitronectin or laminin. On the basis of this substrate requirement of myotube formation, we developed a photolithography-based method to pattern C2C12 myotubes, where myotubes formed exclusively on vitronectin surface patterns. We have determined that the optimal line width to form single myotubes is approximately 30 mum. To illustrate a possible application of this method, we patterned myotubes on the top of commercial substrate-embedded microelectrodes. In contrast to previous experiments where cell patterning was achieved by selective attachment of the cells to patterned surfaces in a medium that contained all of the factors necessary for differentiation, this study illustrates that surface patterning of a signaling molecule, which is essential for skeletal muscle differentiation in a defined system, can result in the formation of aligned myotubes on the patterns. This technique is being developed for applications in cell biology, tissue engineering, and robotics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.