ArticleJournal of virology2007
Canine distemper virus infection requires cholesterol in the viral envelope.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Monoclonal antibody against canine distemper virus H protein potently neutralizes the giant panda-derived strain.AMB Express · 2025Article
- Safety and Immunogenicity of a Canine Distemper DNA Vaccine Formulated with Lipid Nanoparticles in Dogs, Foxes, and Raccoon Dogs.Vaccines · 2025Article
- SARS-CoV-2 entry and fusion are independent of ACE2 localization to lipid rafts.Journal of virology · 2025Article
- Pseudorabies virus upregulates low-density lipoprotein receptors to facilitate viral entry.Journal of virology · 2024Article
- The H protein of attenuated canine distemper virus is degraded via endoplasmic reticulum-associated protein degradation.Frontiers in veterinary science · 2023Article
- Lipid compartments and lipid metabolism as therapeutic targets against coronavirus.Frontiers in immunology · 2023Review
- Overcoming the Barrier of the Respiratory Epithelium during Canine Distemper Virus Infection.mBio · 2022Article
- Evaluation of Newcastle disease virus vaccine effectiveness in dogs with neurological signs of canine distemper.Veterinary research forum : an international quarterly journal · 2022Article
- Roles of Cholesterol in Early and Late Steps of the Nipah Virus Membrane Fusion Cascade.Journal of virology · 2021Article
- Review
- Tropism and molecular pathogenesis of canine distemper virus.Virology journal · 2019Review
- Omega-3 fatty acids, membrane remodeling and cancer prevention.Molecular aspects of medicine · 2018Review
- Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells.Veterinary research · 2018Article
- Cellular cholesterol is required for porcine nidovirus infection.Archives of virology · 2017Article
- Article
- Cholesterol and host cell surface proteins contribute to cell-cell fusion induced by the Burkholderia type VI secretion system 5.PloS one · 2017Article
- Critical role of the lipid rafts in caprine herpesvirus type 1 infection in vitro.Virus research · 2016Article
- Differential effect of cholesterol on type I and II feline coronavirus infection.Archives of virology · 2016Article
- Role of the lipid rafts in the life cycle of canine coronavirus.The Journal of general virology · 2015Article
- Critical role of cellular cholesterol in bovine rotavirus infection.Virology journal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cholesterol is known to play an important role in stabilizing particular cellular membrane structures, so-called lipid or membrane rafts. For several viruses, a dependence on cholesterol for virus entry and/or morphogenesis has been shown. Using flow cytometry and fluorescence microscopy, we demonstrate that infection of cells by canine distemper virus (CDV) was not impaired after cellular cholesterol had been depleted by the drug methyl-beta-cyclodextrin. This effect was independent of the multiplicity of infection and the cellular receptor used for infection. However, cholesterol depletion of the viral envelope significantly reduced CDV infectivity. Replenishment by addition of exogenous cholesterol restored infectivity up to 80%. Thus, we conclude that CDV entry is dependent on cholesterol in the viral envelope. Furthermore, reduced syncytium formation was observed when the cells were cholesterol depleted during the course of the infection. This may be related to the observation that CDV envelope proteins H and F partitioned into cellular detergent-resistant membranes. Therefore, a role for lipid rafts during virus assembly and release as well is suggested.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.