ArticleBMC ophthalmology2007
Molecular characterization, biofilm analysis and experimental biofouling study of Fusarium isolates from recent cases of fungal keratitis in New York State.
Article in BMC ophthalmology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 63 citations in OpenAlex.
- Awareness and Hygiene Practices Among Contact Lens Wearers: A Population-Based Cross-Sectional Survey.Cureus · 2024Article
- The Role of Biofilms in Contact Lens Associated Fungal Keratitis.Antibiotics (Basel, Switzerland) · 2023Article
- Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia.Biomedica : revista del Instituto Nacional de Salud · 2022Article
- Knowledge, attitudes and practices of contact lens use in a South African adolescent population.African health sciences · 2020Article
- Fungal Biofilms: In Vivo Models for Discovery of Anti-Biofilm Drugs.Microbiology spectrum · 2015Article
- Could Histoplasma capsulatum Be Related to Healthcare-Associated Infections?BioMed research international · 2015Review
- Characterization of fusarium keratitis outbreak isolates: contribution of biofilms to antimicrobial resistance and pathogenesis.Investigative ophthalmology & visual science · 2012Article
- Widespread occurrence of diverse human pathogenic types of the fungus Fusarium detected in plumbing drains.Journal of clinical microbiology · 2011Article
- Evaluations of shorter exposures of contact lens cleaning solutions against Fusarium oxysporum species complex and Fusarium solani species complex to simulate inappropriate usage.Antimicrobial agents and chemotherapy · 2011Article
- Article
- A murine model of contact lens-associated fusarium keratitis.Investigative ophthalmology & visual science · 2010Article
- Use of multiple methods for genotyping Fusarium during an outbreak of contact lens associated fungal keratitis in Singapore.BMC infectious diseases · 2008Article
- Fusarium and Candida albicans biofilms on soft contact lenses: model development, influence of lens type, and susceptibility to lens care solutions.Antimicrobial agents and chemotherapy · 2008Article
- Temperature instability of ReNu with MoistureLoc: a new theory to explain the worldwide Fusarium keratitis epidemic of 2004-2006.Transactions of the American Ophthalmological Society · 2008Article
- Phylogenetic diversity and microsphere array-based genotyping of human pathogenic Fusaria, including isolates from the multistate contact lens-associated U.S. keratitis outbreaks of 2005 and 2006.Journal of clinical microbiology · 2007Article
- [Health effects of indoor molds].Wiener medizinische Wochenschrift (1946) · 2007Article
- Fungal keratitis: Mechanisms of infection and management strategies.Survey of ophthalmologyReview
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo characterize Fusarium isolates from recent cases of fungal keratitis in contact lens wearers, and to investigate fungal association with MoistureLoc solution.
methodsWe studied six fungal isolates from recent cases of keratitis in New York State. The isolates were characterized by nucleotide sequencing and phylogenetic analyses of multiple genes, and then typed using minisatellite and microsatellite probes. Experimental fungal biofilm formation was tested by standard methods. MoistureLoc solutions were tested in biofouling studies for their efficacy in elimination of Fusarium contamination.
resultsFusarium solani--corneal ulcers (2 isolates), lens case (1 isolate), and F. oxysporum--corneal ulcer (1 isolate), eye (1 isolate), were recovered from five patients. An opened bottle of MoistureLoc solution provided by a patient also yielded F. solani. Two distinct genotypes of F. solani as well as of F. oxysporum were present in the isolated strains. Remarkably, F. solani strains from the lens case and lens solution in one instance were similar, based on phylogenetic analyses and molecular typing. The solution isolate of F. solani formed biofilm on contact lenses in control conditions, but not when co-incubated with MoistureLoc solution. Both freshly opened and 3-month old MoistureLoc solutions effectively killed F. solani and F. oxysporum, when fungal contamination was simulated under recommended lens treatment regimen (4-hr). However, simulation of inappropriate use (15-60 min) led to the recovery of less than 1% of original inoculum of F. solani or F. oxysporum.
conclusionTemporary survival of F. solani and F. oxysporum in MoistureLoc suggested that improper lens cleaning regimen could be a possible contributing factor in recent infections.
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