ArticleAnalytical chemistry2007
Characterization and resolution of evaporation-mediated osmolality shifts that constrain microfluidic cell culture in poly(dimethylsiloxane) devices.
Article in Analytical chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
What it found
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Who cites it
86 citing papers in PubMed.
- Functional Vascularization in Tumor Organoid and Tumor-on-Chip Models: Evidence Requirements for Attributing Therapy-Resistance Mechanisms.Bioengineering (Basel, Switzerland) · 2026Review
- Atmospheric water harvesting for humidity control in medical devices for global health.Nature biomedical engineering · 2026Review
- Polydimethylsiloxane in Optics.Polymers · 2026Review
- Servo-Actuated 3D-Printed Disposable Microvalves for Automated, Scalable Organoid Culture in Standard Incubators.bioRxiv : the preprint server for biology · 2026Article
- Microfluidics for in vitro fertilization: from science to clinical validation.Human reproduction update · 2026Review
- A modular platform for automated organoid culture and longitudinal imaging.Scientific reports · 2026Article
- Reducing Small Molecule Adsorption in a PDMS-Based Microphysiological System of the Female Reproductive Tract via Parylene-C Coating to Improve Mechanistic Studies.ACS applied materials & interfaces · 2026Article
- Incubator-Free Organoid Culture in a Sealed Recirculatory System.bioRxiv : the preprint server for biology · 2025Article
- Mechanodynamic brain on chip for studying human stem cell derived neuronal networks.Scientific reports · 2025Article
- Zinc eluted from glassware is a risk factor for embryo development in human and animal assisted reproduction†.Biology of reproduction · 2025Article
- A promising breakthrough in pancreatic cancer research: The potential of spheroids as 3D models.BioImpacts : BI · 2025Review
- Integrating computational fluid dynamics into organ-on-chip systems: a glioblastoma-centred design and validation framework.Frontiers in bioengineering and biotechnology · 2025Review
- Article
- Comparative Analysis of In Vitro Pumps Used in Cardiovascular Investigations: Focus on Flow Generation Principles and Characteristics of Generated Flows.Bioengineering (Basel, Switzerland) · 2024Review
- Open-Top Patterned Hydrogel-Laden 3D Glioma Cell Cultures for Creation of Dynamic Chemotactic Gradients to Direct Cell Migration.ACS biomaterials science & engineering · 2024Article
- A Versatile Intestine-on-Chip System for Deciphering the Immunopathogenesis of Inflammatory Bowel Disease.Advanced healthcare materials · 2024Article
- Bioencapsulation of Oocytes and Granulosa Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Design and implementation of a lab-on-a-chip for assisted reproductive technologies.BioImpacts : BI · 2024Article
- Functional PDMS Elastomers: Bulk Composites, Surface Engineering, and Precision Fabrication.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies.Advanced healthcare materials · 2023Review
26 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Evaporation is a critical problem when handling submicroliter volumes of fluids. This paper characterizes this problem as it applies to microfluidic cell culture in poly(dimethylsiloxane) (PDMS) devices and provides a practical solution. Evaporation-mediated osmolality shifts through PDMS membranes with varying thicknesses (10, 1, 0.2, or 0.1 mm) were measured over 96 h. Even in humidified cell culture incubators, evaporation through PDMS and associated shifts in the osmolality of culture media was significant and prevented mouse embryo and human endothelial cell growth and development. A simple diffusion model, where the measured diffusion coefficient for PDMS matches reported values of approximately 10-9 m2/s, accounts for these evaporation and osmolality shifts. To overcome this problem, a PDMS-parylene-PDMS hybrid membrane was developed that greatly suppresses evaporation and osmolality shifts, yet possesses thinness and the flexibility necessary to interface with deformation-based microfluidic actuation systems, maintains the clarity for optical microscopy, and enables the successful development of single-cell mouse embryos into blastocysts under static conditions and culture of human endothelial cells under dynamic recirculation of submicroliter volumes of media. These insights and methods demonstrated specifically for embryo and endothelial cell studies will be generally useful for understanding and overcoming evaporation-associated effects in microfluidic cell cultures.
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Registered trials
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