ArticleLipids2006
Highly unsaturated fatty acid synthesis in marine fish: cloning, functional characterization, and nutritional regulation of fatty acyl delta 6 desaturase of Atlantic cod (Gadus morhua L.).
Article in Lipids, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 220 citations in OpenAlex.
- Trial
- Dietary lipid levels impact lipoprotein lipase, hormone-sensitive lipase, and fatty acid synthetase gene expression in three tissues of adult GIFT strain of Nile tilapia, Oreochromis niloticus.Fish physiology and biochemistry · 2015Trial
- Comparative Response ofAnimals : an open access journal from MDPI · 2026Article
- Hemp Meal (Animals : an open access journal from MDPI · 2026Article
- Biosynthesis of long-chain omega-3 fatty acids by the nestlings of a generalist seabird.The Journal of experimental biology · 2025Article
- Dietary High Levels of Coconut Oil Replacing Fish Oil Did Not Affect Growth, but Promoted Liver Lipid Deposition of Orange-Spotted Groupers (Animals : an open access journal from MDPI · 2024Article
- Efficacy of Palm Oil Application in Tiger Puffer Diets: Growth, Body Composition, Muscle Texture, and Lipid Metabolism.Aquaculture nutrition · 2024Article
- Non-lethal sampling method for the analysis of white muscle fatty acid profiles in European sea bass (Dicentrarchus labrax).Fish physiology and biochemistry · 2023Article
- Genome-Wide RADseq Reveals Genetic Differentiation of Wild and Cultured Populations of Large Yellow Croaker.Genes · 2023Article
- Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp (International journal of molecular sciences · 2023Article
- Genome-wide comparative identification and analysis of membrane-FADS-like superfamily genes in freshwater economic fishes.FEBS open bio · 2023Article
- Effect of Dietary Linoleic Acid (18:2n-6) Supplementation on the Growth Performance, Fatty Acid Profile, and Lipid Metabolism Enzyme Activities of Coho Salmon (Animals : an open access journal from MDPI · 2022Article
- Regulation of Δ6Fads2 Gene Involved in LC-PUFA Biosynthesis Subjected to Fatty Acid in Large Yellow Croaker (Biomolecules · 2022Article
- Growth performance, fatty acid composition, and lipid metabolism are altered in groupers (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Molecular Characterization, Tissue Distribution and Differential Nutritional Regulation of Three n-3 LC-PUFA Biosynthesis-Related Genes in Hybrid Grouper (Animals : an open access journal from MDPI · 2022Article
- Polyunsaturated fatty acids in fish tissues more closely resemble algal than terrestrial diet sources.Hydrobiologia · 2021Article
- Δ6 fatty acid desaturases in polyunsaturated fatty acid biosynthesis: insights into the evolution, function with substrate specificities and biotechnological use.Applied microbiology and biotechnology · 2020Review
- Influence of Parental Fatty Acid Desaturase 2 (Animals : an open access journal from MDPI · 2020Article
- Tissue distribution of transcription for 29 lipid metabolism-related genes in Takifugu rubripes, a marine teleost storing lipid predominantly in liver.Fish physiology and biochemistry · 2020Article
- The Relationship between the Expression of Fatty Acyl Desaturase 2 (Life (Basel, Switzerland) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
This study reports the cloning, functional characterization, tissue expression, and nutritional regulation of a delta6 fatty acyl desaturase of Atlantic cod (Gadus morhua). PCR primers were designed based on the sequences of conserved motifs in available fish desaturases and used to isolate a cDNA fragment from cod liver, with full-length cDNA obtained by rapid amplification of cDNA ends. The cDNA for the putative desaturase was shown to comprise 1980 bp, including a 261-bp 5'-UTR, a 375-bp 3'-UTR, and an ORF of 1344 bp that specified a protein of 447 amino acids. The protein sequence included three histidine boxes, two transmembrane regions, and an N-terminal cytochrome b5 domain containing the heme-binding motif HPGG, all characteristic of microsomal fatty acyl desaturases. The cDNA displayed delta6 desaturase activity in a yeast expression system. Quantitative real-time PCR assay of gene expression in cod showed that the delta6 desaturase gene was expressed highly in brain, to a slightly lesser extent in liver, kidney, intestine, red muscle, and gill, and at much lower levels in white muscle, spleen, and heart. The expression of the delta6 desaturase gene did not appear to be under significant nutritional regulation, with levels in liver and intestine being barely altered in fish fed a vegetable oil blend, in comparison with levels in fish fed fish oil. This was reflected in enzyme activity, as hepatocytes or enterocytes showed very little highly unsaturated FA biosynthesis activity irrespective of diet. Further studies are required to determine why the delta6 desaturase appears to be barely functional in cod under the conditions tested.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.