ArticleDevelopment genes and evolution2007
Gene silencing in the spider mite Tetranychus urticae: dsRNA and siRNA parental silencing of the Distal-less gene.
Article in Development genes and evolution, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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48 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Reciprocal responses in the interaction between Arabidopsis and the cell-content-feeding chelicerate herbivore spider mite.Plant physiology · 2014Pooled it
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- Single-cell sequencing reveals potential novel insights into appendage-patterning and joint-development in a spider.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- RNAi-mediated knockdown of sphingomyelin synthase gene affects egg development and female fecundity in Panonychus citri (McGregor) (Acari: Tetranychidae).Experimental & applied acarology · 2026Article
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- Role of the Hox Genes,International journal of molecular sciences · 2023Article
- Localized efficacy of environmental RNAi in Tetranychus urticae.Scientific reports · 2022Article
- Eggs to long-legs: embryonic staging of the harvestman Phalangium opilio (Opiliones), an emerging model arachnid.Frontiers in zoology · 2022Article
- Molecular characterization, expression, and function of Vitellogenin genes in Phytoseiulus persimilis.Experimental & applied acarology · 2022Article
- Glucosamine-6-phosphate N-acetyltransferase gene silencing by parental RNA interference in rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae).Scientific reports · 2022Article
- An RNA Interference Tool to Silence Genes inInternational journal of molecular sciences · 2022Article
- RNAi gene knockdown in the poultry red mite, Dermanyssus gallinae (De Geer 1778), a tool for functional genomics.Parasites & vectors · 2021Article
- Interactions Between Ticks and Lyme Disease Spirochetes.Current issues in molecular biology · 2021Article
- A genome-wide screening for RNAi pathway proteins in Acari.BMC genomics · 2020Article
- Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response.Scientific reports · 2020Article
- A vegetable oil-based biopesticide with ovicidal activity against the two-spotted spider mite,Engineering in life sciences · 2020Article
- Article
- A Leaf-Mimicking Method for Oral Delivery of Bioactive Substances Into Sucking Arthropod Herbivores.Frontiers in plant science · 2020Article
- Leaf disc-mediated oral delivery of small molecules in the absence of surfactant to the two-spotted spider mite, Tetranychus urticae.Experimental & applied acarology · 2019Article
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2 authors.
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Abstract
A major prerequisite to understanding the evolution of developmental programs includes an appreciation of gene function in a comparative context. RNA interference (RNAi) represents a powerful method for reverse genetics analysis of gene function. However, RNAi protocols exist for only a handful of arthropod species. To extend functional analysis in basal arthropods, we developed a RNAi protocol for the two-spotted spider mite Tetranychus urticae focusing on Distal-less (Dll), a conserved gene involved in appendage specification in metazoans. First, we describe limb morphogenesis in T. urticae using confocal and scanning electron microscopy. Second, we examine T. urticae Dll (Tu-Dll) mRNA expression patterns and correlate its expression with appendage development. We then show that fluorescently labeled double-stranded RNA (dsRNA) and short interfering RNA (siRNA) molecules injected into the abdomen of adult females are incorporated into the oviposited eggs, suggesting that dsRNA reagents can be systemically distributed in spider mites. Injection of longer dsRNA as well as siRNA induced canonical limb truncation phenotypes as well as the fusion of leg segments. Our data suggest that Dll plays a conserved role in appendage formation in arthropods and that such conserved genes can serve as reliable starting points for the development of functional protocols in nonmodel organisms.
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