ArticleMolecular and cellular biology2007
The tripartite motif of nuclear factor 7 is required for its association with transcriptional units.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Genome-wide identification and expression analysis of the B-box gene family in the Apple (Malus domestica Borkh.) genome.Molecular genetics and genomics : MGG · 2018Article
- The RanGTP Pathway: From Nucleo-Cytoplasmic Transport to Spindle Assembly and Beyond.Frontiers in cell and developmental biology · 2015Review
- Association of modified cytosines and the methylated DNA-binding protein MeCP2 with distinctive structural domains of lampbrush chromatin.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Association of the testis-specific TRIM/RBCC protein RNF33/TRIM60 with the cytoplasmic motor proteins KIF3A and KIF3B.Molecular and cellular biochemistry · 2012Article
- Facioscapulohumeral muscular dystrophy region gene 1 is a dynamic RNA-associated and actin-bundling protein.Journal of molecular biology · 2011Article
- Subnuclear targeting of the RNA-binding motif protein RBM6 to splicing speckles and nascent transcripts.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Lampbrush chromosomes enable study of cohesin dynamics.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.Molecular and cellular biology · 2009Article
- The TRIM5alpha B-box 2 domain promotes cooperative binding to the retroviral capsid by mediating higher-order self-association.Journal of virology · 2008Article
- STH2 has 2 B there: An insight into the role of B-box containing proteins in Arabidopsis.Plant signaling & behavior · 2008Article
- Novel domains in the hnRNP G/RBMX protein with distinct roles in RNA binding and targeting nascent transcripts.Nucleus (Austin, Tex.)Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In amphibian oocytes, the maternal nuclear factor NF7 associates with the elongating pre-mRNAs present on the numerous lateral loops of the lampbrush chromosomes. Here, we have purified NF7 from an oocyte extract by using a combination of ion-exchange chromatography and gel filtration chromatography and demonstrated for the first time that nucleoplasmic NF7 exists primarily as free homotrimers. We confirmed the in vivo homotrimerization of NF7 by using a glutaraldehyde cross-linking assay, and we further showed that it only requires the coiled-coil domain of the NF7 tripartite motif/RBCC motif. Interestingly, we also obtained evidence that NF7 is recruited to the nucleus as a homotrimer, and expression of several mutated forms of NF7 in oocytes demonstrated that both the coiled coil and B box of NF7 are required for its chromosomal association. Together, these data strongly suggest that the interaction of NF7 with the active transcriptional units of RNA polymerase II is mediated by a trimeric B box. Finally, and in agreement with a role for NF7 in pre-mRNA maturation, we obtained evidence supporting the idea that NF7 associates with Cajal bodies.
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