ArticleApplied and environmental microbiology2007
High-level production of the low-calorie sugar sorbitol by Lactobacillus plantarum through metabolic engineering.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 124 citations in OpenAlex.
- Development of an in vitro multi-enzyme system for efficient one-pot biosynthesis of sorbitol from fructose-6-phosphate.Bioresources and bioprocessing · 2025Article
- Nutritional Value and Antioxidant Potential of Djiboutian Abundant Seaweeds, With Their Food Applications in Doughnut and Tartare.Food science & nutrition · 2025Article
- Dynamics of Changes in pH and the Contents of Free Sugars, Organic Acids and LAB in Button Mushrooms during Controlled Lactic Fermentation.Foods (Basel, Switzerland) · 2022Article
- Deciphering the Regulation of the Mannitol Operon Paves the Way for Efficient Production of Mannitol in Lactococcus lactis.Applied and environmental microbiology · 2021Article
- Biotechnological Innovations and Therapeutic Application of Pediococcus and Lactic Acid Bacteria: The Next-Generation Microorganism.Frontiers in bioengineering and biotechnology · 2021Review
- Safety Aspects of Genetically Modified Lactic Acid Bacteria.Microorganisms · 2020Review
- Phosphorus-Containing Flame Retardants from Biobased Chemicals and Their Application in Polyesters and Epoxy Resins.Molecules (Basel, Switzerland) · 2019Review
- AcrR and Rex Control Mannitol and Sorbitol Utilization through Their Cross-Regulation of Aldehyde-Alcohol Dehydrogenase (AdhE) in Lactobacillus plantarum.Applied and environmental microbiology · 2019Article
- A review on commercial-scale high-value products that can be produced alongside cellulosic ethanol.Biotechnology for biofuels · 2019Review
- Discovery and Functional Characterization of a Yeast Sugar Alcohol Phosphatase.ACS chemical biology · 2018Article
- d-Alanyl-d-Alanine Ligase as a Broad-Host-Range Counterselection Marker in Vancomycin-Resistant Lactic Acid Bacteria.Journal of bacteriology · 2018Article
- Characterization of the Sorbitol Utilization Cluster of the ProbioticFrontiers in microbiology · 2017Article
- Identification of differential metabolites in liquid diet fermented withAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2016Article
- Proteomic analysis of an engineered isolate of Lactobacillus plantarum with enhanced raffinose metabolic capacity.Scientific reports · 2016Article
- Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum.Microbial cell factories · 2016Article
- Zymomonas mobilis: a novel platform for future biorefineries.Biotechnology for biofuels · 2014Review
- "Direct cloning in Lactobacillus plantarum: electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete".Microbial cell factories · 2012Article
- Lactobacillus plantarum and Lactobacillus buchneri as expression systems: evaluation of different origins of replication for the design of suitable shuttle vectors.Molecular biotechnology · 2012Article
- Metabolic engineering of lactic acid bacteria for the production of industrially important compounds.Computational and structural biotechnology journal · 2012Review
- Elimination of glycerol and replacement with alternative products in ethanol fermentation by Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2011Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sorbitol is a low-calorie sugar alcohol that is largely used as an ingredient in the food industry, based on its sweetness and its high solubility. Here, we investigated the capacity of Lactobacillus plantarum, a lactic acid bacterium found in many fermented food products and in the gastrointestinal tract of mammals, to produce sorbitol from fructose-6-phosphate by reverting the sorbitol catabolic pathway in a mutant strain deficient for both l- and d-lactate dehydrogenase activities. The two sorbitol-6-phosphate dehydrogenase (Stl6PDH) genes (srlD1 and srlD2) identified in the genome sequence were constitutively expressed at a high level in this mutant strain. Both Stl6PDH enzymes were shown to be active, and high specific activity could be detected in the overexpressing strains. Using resting cells under pH control with glucose as a substrate, both Stl6PDHs were capable of rerouting the glycolytic flux from fructose-6-phosphate toward sorbitol production with a remarkably high efficiency (61 to 65% glucose conversion), which is close to the maximal theoretical value of 67%. Mannitol production was also detected, albeit at a lower level than the control strain (9 to 13% glucose conversion), indicating competition for fructose-6-phosphate rerouting by natively expressed mannitol-1-phosphate dehydrogenase. By analogy, low levels of this enzyme were detected in both the wild-type and the lactate dehydrogenase-deficient strain backgrounds. After optimization, 25% of sugar conversion into sorbitol was achieved with cells grown under pH control. The role of intracellular NADH pools in the determination of the maximal sorbitol production is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.