ArticleApplied and environmental microbiology2007
Use of transposon promoter-probe vectors in the metabolic engineering of the obligate methanotroph Methylomonas sp. strain 16a for enhanced C40 carotenoid synthesis.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 51 citations in OpenAlex.
- CRISPRi-based functional genomic screening identifies genes essential for CHScience advances · 2026Article
- Biological conversion of methane to organic molecules: towards a low-carbon bioeconomy.National science review · 2026Review
- All Kinds of Sunny Colors Synthesized from Methane: Genome-Encoded Carotenoid Production byMicroorganisms · 2023Article
- Systems Metabolic Engineering of Methanotrophic Bacteria for Biological Conversion of Methane to Value-Added Compounds.Advances in biochemical engineering/biotechnology · 2022Article
- Optimized Tools and Methods for Methanotroph Genome Editing.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Role of Metabolic Engineering Technologies for the Production of Fatty Acids in Yeast.Biology · 2021Review
- Efficient Counterselection for Methylococcus capsulatus (Bath) by Using a MutatedApplied and environmental microbiology · 2018Article
- Fundamental CRISPR-Cas9 tools and current applications in microbial systems.Synthetic and systems biotechnology · 2017Review
- Methane-Oxidizing Enzymes: An Upstream Problem in Biological Gas-to-Liquids Conversion.Journal of the American Chemical Society · 2016Review
- Bioconversion of methane to lactate by an obligate methanotrophic bacterium.Scientific reports · 2016Article
- Future Applications of Biotechnology to the Energy Industry.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent expansion of genetic and genomic tools for metabolic engineering has accelerated the development of microorganisms for the industrial production of desired compounds. We have used transposable elements to identify chromosomal locations in the obligate methanotroph Methylomonas sp. strain 16a that support high-level expression of genes involved in the synthesis of the C(40) carotenoids canthaxanthin and astaxanthin. with three promoterless carotenoid transposons, five chromosomal locations-the fliCS, hsdM, ccp-3, cysH, and nirS regions-were identified. Total carotenoid synthesis increased 10- to 20-fold when the carotenoid gene clusters were inserted at these chromosomal locations compared to when the same carotenoid gene clusters were integrated at neutral locations under the control of the promoter for the gene conferring resistance to chloramphenicol. A chromosomal integration system based on sucrose lethality was used to make targeted gene deletions or site-specific integration of the carotenoid gene cluster into the Methylomonas genome without leaving genetic scars in the chromosome from the antibiotic resistance genes that are present on the integration vector. The genetic approaches described in this work demonstrate how metabolic engineering of microorganisms, including the less-studied environmental isolates, can be greatly enhanced by identifying integration sites within the chromosome of the host that permit optimal expression of the target genes.
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