ArticleJournal of carcinogenesis2007
Expression and migratory analysis of 5 human uveal melanoma cell lines for CXCL12, CXCL8, CXCL1, and HGF.
Article in Journal of carcinogenesis, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- Identification of novel chemotherapeutic strategies for metastatic uveal melanoma.Scientific reports · 2017Pooled it
- C-X-C Motif Ligand 1 (CXCL1) from melanoma cells down-regulates the invasion of their metastatic melanoma cells.Oncotarget · 2018Article
- Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes.Histochemistry and cell biology · 2018Article
- Genetic determinants of uveal melanoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Review
- Gene expression regulation by agonist-independent constitutive signaling of melanocortin-1 receptor.Endocrinology and metabolism (Seoul, Korea) · 2014Article
- Expression analysis of genes and pathways associated with liver metastases of the uveal melanoma.BMC medical genetics · 2014Article
- Uveal melanoma: Ocular and systemic disease.Saudi journal of ophthalmology : official journal of the Saudi Ophthalmological Society · 2012Article
- Mda-9/syntenin is expressed in uveal melanoma and correlates with metastatic progression.PloS one · 2012Article
- Cancer biomarker discovery: the entropic hallmark.PloS one · 2010Article
- CXCL8 and its cognate receptors in melanoma progression and metastasis.Future oncology (London, England) · 2010Review
- The biology and management of uveal melanoma.Current oncology reports · 2008Review
- In vitro characterization and inhibition of the CXCR4/CXCL12 chemokine axis in human uveal melanoma cell lines.Cancer cell international · 2007Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe aim of this study was to characterize the presence and roles of CXCL12, CXCL8, CXCL1, and HGF in five human uveal melanoma cell lines, using different methods, in order to ascertain their significance in this disease.
methodsFive human uveal melanoma cell lines (92.1, SP6.5, MKT-BR, OCM-1, and UW-1) of known proliferative, invasive, and metastatic potential were used in this experiment. A migration assay was used in order to assess the responsiveness of each cell line towards the four chosen chemotactic factors. Immunohistochemistry was then performed for all five cell lines (cytospins) using antibodies directed toward CXCL1, CXCL8 and their receptors CXCR2 and CXCR1 respectively. Quantitative real-time PCR was then performed on all five cell lines in order to establish the presence of these four chemotactic factors.
resultsAll five human uveal melanoma cell lines migrated towards the four chosen chemotactic factors at a level greater than that of the negative control. Chemokines CXCL1 and CXCL8 resulted in the greatest number of migrating cells in all five of our cell lines. Immunohistochemistry confirmed the expression of CXCL1, CXCL8, and their receptors CXCR2 and CXCR1 in all five of the cell lines. Quantitative real-time PCR results established expression of CXCL8, CXCL1, and HGF in all 5 cell lines tested. CXCL1 and CXCL8 are highly expressed in SP6.5 and UW-1. None of the five cell lines expressed any detectable levels of CXCL12.
conclusionThe migratory ability of the 5 human uveal melanoma cell lines was positively influenced by the four chemotactic factors tested, namely CXCL12, CXCL8, CXCL1, and HGF. Self-expression of chemotactic factors CXCL8, CXCL1, and HGF may indicate an autocrine system, which perhaps contributes to the cells' metastatic ability in vivo.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.