ArticleMicrobiology (Reading, England)2007
Experimental determination of translational starts using peptide mass mapping and tandem mass spectrometry within the proteome of Mycobacterium tuberculosis.
Article in Microbiology (Reading, England), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
- Investigating the role ofMicrobiology spectrum · 2024Article
- StartLink and StartLink+: Prediction of Gene Starts in Prokaryotic Genomes.Frontiers in bioinformatics · 2021Review
- Proteogenomic Analysis and Discovery of Immune Antigens inMolecular & cellular proteomics : MCP · 2017Article
- Biochemical evidence for relaxed substrate specificity of Nα-acetyltransferase (Rv3420c/rimI) of Mycobacterium tuberculosis.Scientific reports · 2016Article
- Article
- Proteogenomic analysis of pathogenic yeast Cryptococcus neoformans using high resolution mass spectrometry.Clinical proteomics · 2014Article
- Analysis of the secretome and identification of novel constituents from culture filtrate of bacillus Calmette-Guerin using high-resolution mass spectrometry.Molecular & cellular proteomics : MCP · 2013Article
- Reannotation of translational start sites in the genome of Mycobacterium tuberculosis.Tuberculosis (Edinburgh, Scotland) · 2013Article
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.Molecular & cellular proteomics : MCP · 2011Article
- A proteogenomic analysis of Shigella flexneri using 2D LC-MALDI TOF/TOF.BMC genomics · 2011Article
- Exploiting proteomic data for genome annotation and gene model validation in Aspergillus niger.BMC genomics · 2009Article
- Experimental determination of translational start sites resolves uncertainties in genomic open reading frame predictions - application to Mycobacterium tuberculosis.Microbiology (Reading, England) · 2009Article
- High accuracy mass spectrometry analysis as a tool to verify and improve gene annotation using Mycobacterium tuberculosis as an example.BMC genomics · 2008Article
- An intragenic distribution bias of DNA uptake sequences in Pasteurellaceae and Neisseriae.Biology direct · 2008Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Identification of protein translation start sites is largely a bioinformatics exercise, with relatively few confirmed by N-terminal sequencing. Translation start site determination is critical for defining both the protein sequence and the upstream DNA which may contain regulatory motifs. It is demonstrated here that translation start sites can be determined during routine protein identification, using MALDI-MS and MS/MS data to select the correct N-terminal sequence from a list of alternatives generated in silico. Applying the method to 13 proteins from Mycobacterium tuberculosis, 11 predicted translational start sites were confirmed, and two reassigned. The authors suggest that these data (be they confirmation or reassignments) are important for the annotation of both this genome and those of organisms with related genes. It was also shown that N-acetylation, reported to be rare in prokaryotes, was present in three of the 13 proteins (23 %), suggesting that in the mycobacteria this modification may be common, and an important regulator of protein function, although more proteins need to be analysed. This method can be performed with little or no additional experimental work during proteomics investigations.
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