Evidence map›Paper›PMID 17259549›Full record

ArticleEukaryotic cell2007

Novel method for preparing spheroplasts from cells with an internal cellulosic cell wall.

Alvin C M Kwok, Carmen C M Mak, Francis T W Wong, Joseph T Y Wong

Open access · diamondAbstract read
In one paragraph

Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.4field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 19 citations in OpenAlex.

  1. Dinochromosome Heterotermini with Telosomal Anchorages.International journal of molecular sciences · 2024
    Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Knockdown of Dinoflagellate Cellulose SynthaseFrontiers in microbiology · 2019
    Article
  7. Genetic Engineering ofFrontiers in microbiology · 2018
    Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Alvin C M KwokDepartment of Biology, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, People's Republic of China.
Carmen C M Mak
Francis T W Wong
Joseph T Y Wong
Hong Kong University of Science and Technology · HK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protoplast and spheroplast preparations allow the transfer of macromolecules into cells and provide the basis for the generation of engineered organisms. Crypthecodinium cohnii cells harvested from polyethylene glycol-containing agar plates possessed significantly lower levels of cellulose in their cortical layers, which facilitated the delivery of fluorescence-labeled oligonucleotides into these cells.

Indexed as

Organisms, Genetically ModifiedAnimalsCelluloseCell WallDinoflagellidaG1 PhaseG2 PhaseMembrane FluidityPermeabilityPolyethylene GlycolsProtoplastsSpheroplastsStaining and LabelingCellulosePolyethylene Glycols

Identifiers

PMID17259549
PMCPMC1828928
OpenAlexW2125552861

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.