ArticleEukaryotic cell2007
Copine A is required for cytokinesis, contractile vacuole function, and development in Dictyostelium.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Loss of Copine D Leads to Ras Activation inCells · 2026Article
- Loss of Copine D Leads to Ras Activation inbioRxiv : the preprint server for biology · 2026Article
- Copine A is essential for calcium homeostasis in Dictyostelium.Cell calcium · 2025Article
- Copine C plays a role in adhesion and streaming inCell adhesion & migration · 2024Article
- Review
- Phosphatidylserine exposure promotes increased adhesion in Dictyostelium Copine A mutants.PloS one · 2021Article
- Copine A regulates the size and exocytosis of contractile vacuoles and postlysosomes in Dictyostelium.FEBS open bio · 2020Article
- Calmodulin and Calmodulin Binding Proteins inInternational journal of molecular sciences · 2020Review
- Differential expression and subcellular localization of Copines in mouse retina.The Journal of comparative neurology · 2019Article
- Article
- Cyclic AMP signaling in Dictyostelium promotes the translocation of the copine family of calcium-binding proteins to the plasma membrane.BMC cell biology · 2018Article
- Dissection of Francisella-Host Cell Interactions in Dictyostelium discoideum.Applied and environmental microbiology · 2015Article
- Two-pore channel 1 interacts with citron kinase, regulating completion of cytokinesis.Channels (Austin, Tex.) · 2015Article
- CPNA-1, a copine domain protein, is located at integrin adhesion sites and is required for myofilament stability in Caenorhabditis elegans.Molecular biology of the cell · 2013Article
- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- Copine A is expressed in prestalk cells and regulates slug phototaxis and thermotaxis in developing Dictyostelium.Development, growth & differentiation · 2011Article
- Requirement of calcium binding, myristoylation, and protein-protein interaction for the Copine BON1 function in Arabidopsis.The Journal of biological chemistry · 2010Article
- Copine A plays a role in the differentiation of stalk cells and the initiation of culmination in Dictyostelium development.BMC developmental biology · 2010Article
- Differential protein expression in Spirometra erinacei according to its development in its final host.Parasitology research · 2009Article
- Entamoeba histolytica modulates a complex repertoire of novel genes in response to oxidative and nitrosative stresses: implications for amebic pathogenesis.Cellular microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copines make up a family of soluble, calcium-dependent, membrane binding proteins found in a variety of eukaryotic organisms. In an earlier study, we identified six copine genes in the Dictyostelium discoideum genome and focused our studies on cpnA. Our previous localization studies of green fluorescent protein-tagged CpnA in Dictyostelium suggested that CpnA may have roles in contractile vacuole function, endolysosomal trafficking, and development. To test these hypotheses, we created a cpnA- knockout strain, and here we report the initial characterization of the mutant phenotype. The cpnA- cells exhibited normal growth rates and a slight cytokinesis defect. When placed in starvation conditions, cpnA- cells appeared to aggregate into mounds and form fingers with normal timing; however, they were delayed or arrested in the finger stage. When placed in water, cpnA- cells formed unusually large contractile vacuoles, indicating a defect in contractile vacuole function, while endocytosis and phagocytosis rates for the cpnA- cells were similar to those seen for wild-type cells. These studies indicate that CpnA plays a role in cytokinesis and contractile vacuole function and is required for normal development, specifically in the later stages prior to culmination. We also used real-time reverse transcription-PCR to determine the expression patterns of all six copine genes during development. The six copine genes were expressed in vegetative cells, with each gene exhibiting a distinct pattern of expression throughout development. All of the copine genes except cpnF showed an upregulation of mRNA expression at one or two developmental transitions, suggesting that copines may be important regulators of Dictyostelium development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.