ArticleApplied and environmental microbiology2007
Development of a new, combined rapid method using phage and PCR for detection and identification of viable Mycobacterium paratuberculosis bacteria within 48 hours.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 83 citations in OpenAlex.
- Phage amplification-coupled CRISPR/Cas12a system for selective detection of viableFrontiers in microbiology · 2026Article
- Bacteriophages as a modern diagnostic tool: innovations, applications and challenges.Molecular biology reports · 2025Review
- Evaluation of the reproducibility and performance characteristics of the Phagomagnetic separation-qPCR assay for rapidly detecting viableFrontiers in veterinary science · 2025Article
- Utilisation of Actiphage in combination with ISJournal of veterinary research · 2023Article
- Mycobacteriophages in diagnosis and alternative treatment of mycobacterial infections.Frontiers in microbiology · 2023Review
- A Review on Mycobacteriophages: From Classification to Applications.Pathogens (Basel, Switzerland) · 2022Review
- Quantitative Imaging of Bacteriophage Amplification for Rapid Detection of Bacteria in Model Foods.Frontiers in microbiology · 2022Article
- Development of a reference standard for the detection and quantification of Mycobacterium avium subsp. paratuberculosis by quantitative PCR.Scientific reports · 2021Article
- MAP, Johne's disease and the microbiome; current knowledge and future considerations.Animal microbiome · 2021Review
- Phage Amplification Assay for Detection of Mycobacterial Infection: A Review.Microorganisms · 2021Review
- Application of the Actiphage® Assay to Detect ViableFrontiers in veterinary science · 2021Article
- Bacteriophage-Based Methods for Detection of ViableFrontiers in veterinary science · 2021Review
- The Application of Bacteriophage Diagnostics for Bacterial Pathogens in the Agricultural Supply Chain: From Farm-to-Fork.PHAGE (New Rochelle, N.Y.) · 2020Review
- A novel one-day phage-based test for rapid detection and enumeration of viable Mycobacterium avium subsp. paratuberculosis in cows' milk.Applied microbiology and biotechnology · 2020Article
- Methods for detection of viable foodborne pathogens: current state-of-art and future prospects.Applied microbiology and biotechnology · 2020Review
- The development and use of ActiphageMicrobial biotechnology · 2020Article
- Phage-Mediated Molecular Detection (PMMD): A Novel Rapid Method for Phage-Specific Bacterial Detection.Viruses · 2020Article
- Estimation of Performance Characteristics of Analytical Methods forFrontiers in microbiology · 2019Article
- In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions.BMC microbiology · 2018Article
- Contribution of Fluorescence Techniques in Determining the Efficiency of the Non-thermal Plasma Treatment.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The FASTPlaqueTB assay is an established diagnostic aid for the rapid detection of Mycobacterium tuberculosis from human sputum samples. Using the FASTPlaqueTB assay reagents, viable Mycobacterium avium subsp. paratuberculosis cells were detected as phage plaques in just 24 h. The bacteriophage used does not infect M. avium subsp. paratuberculosis alone, so to add specificity to this assay, a PCR-based identification method was introduced to amplify M. avium subsp. paratuberculosis-specific sequences from the DNA of the mycobacterial cell detected by the phage. To give further diagnostic information, a multiplex PCR method was developed to allow simultaneous amplification of either M. avium subsp. paratuberculosis or M. tuberculosis complex-specific sequences from plaque samples. Combining the plaque PCR technique with the phage-based detection assay allowed the rapid and specific detection of viable M. avium subsp. paratuberculosis in milk samples in just 48 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.