ArticlePlant physiology2007
Characterization of a plastid triacylglycerol lipase from Arabidopsis.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 74 citations in OpenAlex.
- Cryo-SEM and large volume FIB-SEM of Arabidopsis cotyledons: Degradation of lipid bodies, biogenesis of glyoxysomes and reorganisation of organelles during germination.Journal of microscopy · 2025Article
- Inducible expression of DEFECTIVE IN ANTHER DEHISCENCE 1 enhances triacylglycerol accumulation and lipid droplet formation in vegetative tissues.The Plant journal : for cell and molecular biology · 2025Article
- A maize triacylglycerol lipase inhibits sugarcane mosaic virus infection.Plant physiology · 2022Article
- Two Triacylglycerol Lipases Are Negative Regulators of Chilling Stress Tolerance in Arabidopsis.International journal of molecular sciences · 2022Article
- Developing Climate-Resilient Chickpea Involving Physiological and Molecular Approaches With a Focus on Temperature and Drought Stresses.Frontiers in plant science · 2019Review
- Effect of salt stress on fatty acid and α-tocopherol metabolism in two desert shrub species.Planta · 2018Article
- A High Temperature-Dependent Mitochondrial Lipase EXTRA GLUME1 Promotes Floral Phenotypic Robustness against Temperature Fluctuation in Rice (Oryza sativa L.).PLoS genetics · 2016Article
- Ectopic Expression of WRINKLED1 Affects Fatty Acid Homeostasis in Brachypodium distachyon Vegetative Tissues.Plant physiology · 2015Article
- Living to Die and Dying to Live: The Survival Strategy behind Leaf Senescence.Plant physiology · 2015Review
- Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications.International journal of molecular sciences · 2015Review
- Article
- Wound-induced expression of DEFECTIVE IN ANTHER DEHISCENCE1 and DAD1-like lipase genes is mediated by both CORONATINE INSENSITIVE1-dependent and independent pathways in Arabidopsis thaliana.Plant cell reports · 2014Article
- Change in membrane fatty acid compositions and cold-induced responses in chickpea.Molecular biology reports · 2013Article
- Acyl-lipid metabolism.The arabidopsis book · 2013Article
- Transcriptome analysis of yellow horn (Xanthoceras sorbifolia Bunge): a potential oil-rich seed tree for biodiesel in China.PloS one · 2013Article
- The Arabidopsis sn-1-specific mitochondrial acylhydrolase AtDLAH is positively correlated with seed viability.Journal of experimental botany · 2011Article
- Chemical inhibitors of monogalactosyldiacylglycerol synthases in Arabidopsis thaliana.Nature chemical biology · 2011Article
- Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: pathway description and gene discovery for production of next-generation biofuels.BMC genomics · 2011Article
- A Brassica napus lipase locates at the membrane contact sites involved in chloroplast development.PloS one · 2011Article
- FIBRILLIN4 is required for plastoglobule development and stress resistance in apple and Arabidopsis.Plant physiology · 2010Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Full-length cDNA corresponding to Arabidopsis (Arabidopsis thaliana) gene At2g31690, which has been annotated in GenBank as a putative triacylglycerol (TAG) lipase, was obtained by reverse transcription-polymerase chain reaction using RNA from senescing rosette leaves of Arabidopsis as a template. The cognate protein was found to contain the lipase active site sequence, and corresponding recombinant protein proved capable of deesterifying TAG. In vitro chloroplast import assays indicated that the lipase is targeted to chloroplasts. This was confirmed by confocal microscopy of rosette leaf tissue treated with fluorescein isocyanate-labeled, lipase-specific antibody, which revealed that lipase protein colocalizes with plastoglobular neutral lipids. Western-blot analysis indicated that the lipase is expressed in roots, inflorescence stems, flowers, siliques, and leaves and that it is strongly up-regulated in senescing rosette leaf tissue. Transgenic plants with suppressed lipase protein levels were obtained by expressing At2g31690 cDNA in antisense orientation under the regulation of a constitutive promoter. Transgenic plants bolted and flowered at the same time as wild-type plants, but were severely stunted and exhibited delayed rosette senescence. Moreover, the stunted growth phenotype correlated with irregular chloroplast morphology. The chloroplasts of transgenic plants were structurally deformed, had reduced abundance of thylakoids that were abnormally stacked, and contained more plastoglobular neutral lipids than chloroplasts of wild-type plants. These observations collectively indicate that this TAG lipase plays a role in maintaining the structural integrity of chloroplasts, possibly by mobilizing the fatty acids of plastoglobular TAG.
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