ArticleNucleic acids research2007
Reversible site-specific tagging of enzymatically synthesized RNAs using aldehyde-hydrazine chemistry and protease-cleavable linkers.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Aptamers: precision tools for diagnosing and treating infectious diseases.Frontiers in cellular and infection microbiology · 2024Review
- Efficient post-synthesis incorporation and conjugation of reactive ketones in RNAChemical communications (Cambridge, England) · 2023Article
- Aptamer Therapeutics in Cancer: Current and Future.Cancers · 2018Review
- Dark Hydrazone Fluorescence Labeling Agents Enable Imaging of Cellular Aldehydic Load.ACS chemical biology · 2016Article
- Covalent adduct formation between the antihypertensive drug hydralazine and abasic sites in double- and single-stranded DNA.Chemical research in toxicology · 2014Article
- Novel modifications in RNA.ACS chemical biology · 2012Review
- Bisaryl hydrazones as exchangeable biocompatible linkers.Angewandte Chemie (International ed. in English) · 2010Article
- A new usage of functionalized oligodeoxynucleotide probe for site-specific modification of a guanine base within RNA.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
The investigation of RNA structure, dynamics and biological function often requires the site-specific incorporation of non-natural moieties. Here we describe the functionalization of RNA transcripts by aldehyde-hydrazine chemistry using a simple initiator nucleotide that carries an acetal-protected aldehyde function. This initiator nucleotide was efficiently incorporated into RNA, and the modified RNAs were quantitatively coupled to a peptide derivative displaying a hydrazine moiety at one end, a biotin tag at the other, and a trypsin-cleavable sequence in between. RNA conjugates could be easily isolated by affinity chromatography on streptavidin agarose and quantitatively cleaved off the support by trypsin treatment without detectable RNA degradation. The strategy described here may allow the incorporation of various new features into enzymatically synthesized RNA under mild conditions.
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Registered trials
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