ArticleResearch in microbiology
Nocardia asteroides strain GUH-2 induces proteasome inhibition and apoptotic death of cultured cells.
Article in Research in microbiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Molecular mechanisms underlyingFrontiers in cellular and infection microbiology · 2026Review
- Advances in the molecular pathogenesis ofFrontiers in cellular and infection microbiology · 2026Review
- An isocitrate lyase gene-deleted strain ofFrontiers in veterinary science · 2025Article
- Biological characteristics and pathogenicity comparison ofFrontiers in veterinary science · 2024Article
- Isolation and Characterization of Nocardiae Associated with Foaming Coastal Marine Waters.Pathogens (Basel, Switzerland) · 2021Article
- Function and Characterization of an Alanine Dehydrogenase Homolog FromFrontiers in veterinary science · 2021Article
- Mce1C and Mce1D facilitate N. farcinica invasion of host cells and suppress immune responses by inhibiting innate signaling pathways.Scientific reports · 2020Article
- Nfa34810 FacilitatesInfection and immunity · 2020Article
- Virulence test using nematodes to prescreenPeerJ · 2017Article
- Article
- Nocardia asteroides-Induced movement abnormalities in mice: Relevance for Parkinson's disease?Movement disorders : official journal of the Movement Disorder Society · 2016Review
- The Nocardia cyriacigeorgica GUH-2 genome shows ongoing adaptation of an environmental Actinobacteria to a pathogen's lifestyle.BMC genomics · 2013Article
- Nocardia farcinica activates human dendritic cells and induces secretion of interleukin-23 (IL-23) rather than IL-12p70.Infection and immunity · 2012Article
- Natural toxins implicated in the development of Parkinson's disease.Therapeutic advances in neurological disorders · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Many bacterial pathogens have the ability to induce apoptosis in their hosts. It was previously shown that Nocardia asteroides strain GUH-2, a Gram-positive facultatively intracellular pathogen, is capable of inducing the apoptotic death of dopaminergic cells in the murine brain and in PC12 cells, a rat cell line. In this study, the apoptosis-inducing potential of N. asteroides GUH-2 was further explored using HeLa cells, a human epithelial cell line. HeLa cells were incubated for 5h with live nocardiae, heat-killed bacteria, or unconcentrated nocardial culture filtrate, and changes to the cells were monitored. Consistent with the previous studies, N. asteroides GUH-2 induced DNA fragmentation and apoptosis in HeLa cells. Caspase activation and disruption of the mitochondrial membrane potential were also investigated to determine their roles in the induction of cell death. In all these experiments, significant changes were only induced by live nocardiae. A recent publication demonstrated that systemic administration of proteasome inhibitors can induce a Parkinsonian syndrome in rats that includes intraneuronal inclusions and characteristic behavioral alterations. Similar effects have been observed in mice and monkeys infected with N. asteroides GUH-2. In addition, some reports have shown that proteasome inhibition causes apoptotic death of affected cells. We therefore investigated the ability of N. asteroides GUH-2 to inhibit proteasome activity. Proteasome activity was significantly reduced, suggesting that this mechanism may be involved in the induction of apoptosis by these bacteria.
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