ArticleThe American journal of pathology2007
Hepatitis C virus replication in transfected and serum-infected cultured human fetal hepatocytes.
Article in The American journal of pathology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 90 citations in OpenAlex.
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- LIN28 and histone H3K4 methylase induce TLR4 to generate tumor-initiating stem-like cells.iScience · 2023Article
- Hepatitis D virus (HDV): investigational therapeutic agents in clinical trials.Expert opinion on investigational drugs · 2022Review
- Generation of metabolically functional hepatocyte-like cells from dedifferentiated fat cells by Foxa2, Hnf4a and Sall1 transduction.Genes to cells : devoted to molecular & cellular mechanisms · 2020Article
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- A murine model demonstrating reversal of structural and functional correlates of cirrhosis with progenitor cell transplantation.Scientific reports · 2019Article
- Germline genetic variants with implications for disease risk and therapeutic outcomes.Physiological genomics · 2017Review
- Are all cancer stem cells created equal?Stem cells translational medicine · 2014Review
- Osteopontin is up-regulated in chronic hepatitis C and is associated with cellular permissiveness for hepatitis C virus replication.Clinical science (London, England : 1979) · 2014Article
- Interferon lambda: opportunities, risks, and uncertainties in the fight against HCV.Frontiers in immunology · 2014Review
- Cancer stem cells in the development of liver cancer.The Journal of clinical investigation · 2013Review
- Modeling hepatitis C virus infection using human induced pluripotent stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Development of persistent HCV genotype 3a infection cell culture model in huh-7 cell.Virology journal · 2012Article
- In vitro systems for the study of hepatitis C virus infection.International journal of hepatology · 2012Article
- Hepatitis C virus induces interferon-λ and interferon-stimulated genes in primary liver cultures.Hepatology (Baltimore, Md.) · 2011Article
- Expression of paramyxovirus V proteins promotes replication and spread of hepatitis C virus in cultures of primary human fetal liver cells.Hepatology (Baltimore, Md.) · 2011Article
- Hepatitis C virus-induced cancer stem cell-like signatures in cell culture and murine tumor xenografts.Journal of virology · 2011Article
- Inhibition of HCV 3a genotype entry through host CD81 and HCV E2 antibodies.Journal of translational medicine · 2011Article
- IFN-λs.Current opinion in immunology · 2011Review
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9 authors at 1 institution in 1 country.
Funding
Abstract
Understanding the pathogenesis of hepatitis C requires the availability of tissue culture models that sustain viral replication and produce infectious particles. We report on the establishment of a culture system of nontransformed human fetal hepatocytes that supports hepatitis C virus (HCV) replication after transfection with full-length in vitro-transcribed genotype 1a HCV RNA without adaptive mutations and infection with patient sera of diverse HCV genotypes. Transfected and infected hepatocytes expressed HCV core protein and HCV negative-strand RNA. For at least 2 months, transfected or infected cultures released HCV into the medium at high levels and usually with a cyclical pattern. Viral replication had some cytotoxic effects on the cells, which produced interferon (IFN)-beta as a component of the antiviral response. Medium from transfected cells was able to infect naïve cultures in a Transwell system, and the infection was blocked by IFN-alpha and IFN-lambda. Viral particles analyzed by sucrose density centrifugation had a density of 1.17 g/ml. Immunogold labeling with antibody against HCV envelope protein E2 decorated the surface of the viral particles, as visualized by electron microscopy. This culture system may be used to study the responses of nontransformed human hepatocytes to HCV infection, to analyze serum infectivity, and to clone novel HCVs from infected patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.